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Volume 46(1); March 2014

Articles

Abscisic acid (ABA) is a stress hormone that functions in abiotic stress adaptation in plants. Thus many efforts have been made to identify the molecular mechanisms of ABA signal transduction pathways. Recently there were big advances in understanding molecular mechanisms of ABA dependent expression. From the ABA receptors to the transcription factors, signaling components were discovered and the biological networks among the components were identified. In this review, we describe the ABA signaling components and the rice orthologues identified. These show that signaling network systems of ABA are highly conserved in dicot and monocot plants and we are able to manipulate the ABA signaling components to develop the abiotic stress tolerant crops.

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Biomass Production in the Short Rotation Coppice of Poplar Species Treated with Low-Concentrated Liquid Fertilizer
Hyun-Chul Kim1, Hanna Shin1, Heon-Ho Lee2, Jin-Kie Yeo3, and Kyu-Suk Kang4*
Korean. J. Breed. Sci. ;46(1):10-16.   Published online March 31, 2014
DOI: https://doi.org/10.9787/KJBS.2014.46.1.010
A short rotation coppice (SRC) refers to cultivation systems using fast-growing tree species with the ability to re-sprout from the stump after harvest. Harvest in SRC cultivation occurs in short intervals, 2-6 years, and management practices are more similar to those of agricultural annual crops than to forestry. The SRC for production of biomass for heat and electricity is considered a very promising means to meet the different targets set to increase the amount of renewable energy. This study was conducted to analyze growth characteristics and biomass production of poplar clones in the SRC under the treatment of slurry composting and biofiltration liquid fertilizer (SCBLF). The average survival rate of poplar clones at SCBLF treatment was 95.0% and that at control was 92.5%, respectively. The average number of shoot at the treatment was 11.8 ea/clone and that of control was 11.5 ea/clone. ‘72-31’, ‘Bonghwa1’ and ‘Clivus’ from Populus alba × P. glandulosa were superior clones for the number of shoot with 17.1 ea/clone, 14.5 ea/clone and 13.8 ea/clone at the treatment of SCBLF. For the average of leaf area, the SCBLF treatment showed 35% broader leaf (71.0 cm2) than control (52.3 cm2). The annual average of above-ground biomass production showed 51% better at the SCBLF treatment (8.5 ton/ha) than control (5.6 ton/ha). ‘Clivus’, ‘72-31’ and ‘Bonghwa1’ clones from P. alba × P. glandulosa were superior for the annual average of above-ground biomass production with 15.2 ton/ha, 14.0 ton/ha and 11.6 ton/ha at the treatment of SCBLF.
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Genetic Analysis on the Blast Resistance Gene of ‘Suweon506’ Derived from a Wild Relative, Oryza minuta
Ji Ung Jeung*, Byung Joo Choi, Kyung Ho Kang, Young Jun Mo, Sang Bok Lee, Sea Kwan Oh, and Bo Kyeong Kim
Korean. J. Breed. Sci. ;46(1):17-27.   Published online March 31, 2014
DOI: https://doi.org/10.9787/KJBS.2014.46.1.017
Rice blast is one of the major threats to stable rice production. In modernized rice breeding system, development of rice cultivars harboring resistance gene is one of the most efficient approaches against blast disease. Wild rice species, to the context, have been recognized as valuable genetic resources in improving resistance or tolerance level of modern commercial rice cultivars against biotic or abiotic stresses. National Institute of Crop Science (NICS), Korea developed an introgression line, ‘Suweon 506’, which has inherited the rice blast resistance from a BBCC genome wild Oryza species, O. minuta (Acc. 101141). Genetic analysis was carried out to localize the chromosomal region responsible for the rice blast resistance of ‘Suweon 506’ by using F2 progenies from ‘Suweon 506’ × ‘Milyang 23’. Association analysis between marker genotypes and their inferred resistance levels of F2 progenies against a Korean blast isolate, ‘93-072’ localized the target genetic region on rice chromosome 12. Further association analysis with increased number of DNA markers, and e-Landings on the rice pseudomolecule 6, the segment of ‘RM101-S10704-RM1337’ was identified to be tightly linked to the rice blast resistance gene from O. minuta at the 8.8~11.9 Mbp physical region of rice chromosome 12, where at least 9 rice blast resistance genes have been also identified. The 3.1 Mb equivalent virtual contig, composed with 31 BAC/PAC clones will be further analyzed for fine mapping and gene identification.
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Analysis of Genetic Relationship of Seedless Germplasm and Validation Assay of the P3_VvAGL11 Marker Linked to Seedlessness in Grapevines
Youn Young Hur1, Chan Jin Jung1, Jung-Ho Noh1, Sung-Min Jung1, Jong-Chul Nam1, Kyung Ho Ma2, and Kyo-Sun Park1*
Korean. J. Breed. Sci. ;46(1):28-36.   Published online March 31, 2014
DOI: https://doi.org/10.9787/KJBS.2014.46.1.028
The genetic relationships of twenty seedless grape cultivars, used as parents for seedless grape breeding, were analyzed and the validation assay of the P3_VvAGL11 marker related to the seedlessness was examined. Total 218 polymorphic bands were generated by 30 SSR markers. The average number of alleles per marker was 7.3. The polymorphism information contents (PIC) averaged 0.442, value ranging from 0.052 to 0.083 by each SSR marker. Eighteen seedless grape cultivars derived from ‘Sultanina’ and ‘Kishmish Chernyi’ had 196-bp alleles of the P3_VvAGL11 associated with seedlessness. The unweighted pair-group method arithmetic average (UPGMA) cluster analysis was performed using selected 218 polymorphic bands. And four groups were derived on the similarity index 0.722. Each group accorded with the classification by the ratio of the European species (Vitis vinifera), the American species (V. labrusca) and the inter-specific hybrid between the two species (V. labruscana). Similarity values among the tested seedless grape cultivars were ranged from 0.592 for ‘Beauty Seedless’ and ‘Honey Seedless’ to 0.844 for ‘Emerald Seedless’ and ‘Ruby Seedless’, the average similarity value was 0.715. These results could be used as useful reference for selecting parents with diverse backgrounds and early selection of seedless seedlings in breeding program of seedless grape cultivars.
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Screening of Lycopene-rich Germplasms using Microplate Method in Watermelon (Citrullus Lanatus (thunb.) Matsum. & Nakai)
Woo-Moon Lee, Min Jung Kwon, Lee-Seul Song, Su Kim*, Hee Ju Lee, Eun-Young Yang, Hak-Soon Choi, Yun-Chan Huh, Dong Kum Park, and Moo kyoung Yoon
Korean. J. Breed. Sci. ;46(1):37-43.   Published online March 31, 2014
DOI: https://doi.org/10.9787/KJBS.2014.46.1.037
Recent researches focused on breeding lycopene–rich watermelon varieties are active since lycopene content in watermelon is 60% higher than in tomato. We developed a new microplate method using a UV spectrophotometer to select genetic resources of watermelon containing high lycopene content. The microplate method showed more accurate analysis (about 97% positive correlation) than HPTLC, suggesting the microplate method is a reliable analytical technique for the rapid and simple selection of watermelon resources. Lycopene contents of 96 watermelon accessions using the new microplate method was a wide variation of 3.2-98.3 μg.g-1FW to assess, allowing selection of 15 high-lycopene watermelon accessions. Lycopene contents of the 15 watermelon accessions was 4.4~9.6 fold higher than lycopene content of a commercial cultivar ‘Speedplus’. These results suggest that the watermelon accessions selected using a novel microplate method can be used for breeding of lycopene-rich cultivars.
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Assessment of Gene Flow in Disease Resistant (OsCK1) Genetically Modified Rice
Sung-Dug Oh1, Myung-Ho Lim1, Tae-Hun Ryu1, Soo-In Sohn1, Soon Ki Park2, and Kijong Lee1*
Korean. J. Breed. Sci. ;46(1):44-51.   Published online March 31, 2014
DOI: https://doi.org/10.9787/KJBS.2014.46.1.044
Although genetically modified (GM) crops have never been cultivated commercially in Korea, it is necessary for a thorough assessment of the risks associated with their environmental release. We determined the frequency of pollen mediated gene flow from disease resistant GM rice (OsCK1) to non-GM rice (Nagdongbyeo) and weedy rice (R55). A total of 449,711 or 164,604 seeds were collected from non-GM and weedy rice, respectively which were planted around OsCK1. Resistance of the hybrids was determined by repeated spraying of herbicide and DNA analysis using specific primer to confirm hybrids. Though non-GM rice and weedy rice have similar flowering time, the hybrids were found only in non-GM rice and out-crossing ranged from 0.018% at 0.3 m to 0.013% at 0.6 m. All of hybrids were located within 0.6 m distance from the GM rice plot in southerly direction. The meteorological factors including temperature and relative humidity during flowering time were found to be the most important factors for determining rice out-crossing. It should be considered many factors like the local weather condition and flowering time to set up the safety management policy to prevent pollen mediated gene flow between GM and conventional crop.
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Screening of Soybean Germplasm with High Starch Content
Sung Cheol Koo, Myeong Gi Jeon, Young Hoon Lee, Hyun-Young Kim, Beom Kyu Kang, Jong Min Go,In Youl Baek, Hong Tai Yun, In Youl Baek, and Man Soo Choi*
Korean. J. Breed. Sci. ;46(1):52-57.   Published online March 31, 2014
DOI: https://doi.org/10.9787/KJBS.2014.46.1.052
Starch is the most important carbohydrate in the human and animal diets and it also has numerous industrial applications. Starch is widely used as a key material for processed foods such as bread, pancakes, cereals, noodles, pasta, porridge and tortilla. Increasing the starch content in food crops is one of the important targets for crop breeding. We used a starch-iodine test to identify varieties with high starch content in 2354 soybean germplasm collection and found a total of 126 germplasm that showed a strongly positive reaction. We quantified starch content from highly stained 7 soybean accessions by standard method (glucose-oxidase). As results, starch contents of 7 soybean germplasm were 2.81~4.55%, whereas the weakly stained controls showed low starch content with less than 1%. We also measured the contents of protein, fatty acid, water potential and free sugar in 7 soybean germplasm with high starch content. High starch germplasm showed lower protein content, while low starch germplasm showed higher protein content. Our results suggest that the increasing of starch content could affect protein level in soybean seed.
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Construction of SSR Profile Database for Variety Identification of Blueberry in Korea
Jee-Hwa Hong1*, Yong-Sham Kwon2, Young Kim3, Eun-Ju Kim4, Eun-Hee Soh1, and Keun-Jin Choi5
Korean. J. Breed. Sci. ;46(1):58-65.   Published online March 31, 2014
DOI: https://doi.org/10.9787/KJBS.2014.46.1.058
Blueberry (Vaccinium spp.) is a member of Ericaceae and the recent important small fruit crop. This study was to construct a DNA marker database for blueberry varieties in Korea using simple sequence repeat (SSR) markers. A set of 49 SSR primer pairs was tested to select polymorphic SSR marker in 6 varieties. With 49 primer pairs, seventeen primer pairs showed polymorphism, reproducibility and band clearance. The genetic relationship among 34 varieties by using 17 SSR markers was analyzed. A total of 115 polymorphic amplified fragments were obtained by 17 SSR markers. Two to fifteen SSR alleles were detected for each locus with an average of 6.8 alleles per locus. Average polymorphism information content (PIC) was 0.671, ranging from 0.248 to 0.888. A total of 115 SSR marker loci were used to calculate Jaccard’s distance coefficients for cluster analysis by unweighted pair-group method with arithmetical average (UPGMA). Genetic distance ranged from 0.31 to 0.81 in 34 varieties and the dendrogram at a similarity 0.40 gave 3 main clusters according to blueberry species. Totally 34 varieties were identified by 17 SSR markers. Out of 17 SSR markers, a set of 3 minimum SSR markers was also enabled the identification of 34 varieties. Hence, we concluded that these SSR markers will be available for identifying blueberry varieties and alternative choice to distinctness, uniformity, stability (DUS) examination in blueberry along with valid phenotypic data.
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Evaluation of Forage Yield and Quality for the Accessions Derived from Inter-specific Cross between Wild and Cultivated Soybeans
E. J. Lee1, H. J. Choi1, D. H. Shin2, C. H. Kwon3, J. G. Shannon4, and J. D. Lee2*
Korean. J. Breed. Sci. ;46(1):66-77.   Published online March 31, 2014
DOI: https://doi.org/10.9787/KJBS.2014.46.1.066
This study was conducted to evaluate forage yield and quality for the accessions derived from inter-specific cross between wild and cultivated soybeans. First of all, three soybean lines (W02, W09 and W11) derived from PI483463 (Glycine soja) x Hutcheson (G. max) and three cultivated soybeans (Daewon, Pungsannamul and Bosug) were evaluated to determine forage yield and quality at the R2 (full bloom stage), R4 (full pod stage) and R6 (full seed stage) stages of development. Based on forage yield and quality, R6 was determined the optimal harvest stage to provide forage of high quality and adequate quantity for animals in both lines derived from G. soja × G. max and cultivated soybean. Second, 25 selected lines from PI483463 × Hutcheson or S-100 (G. max) × PI483463 and four common seed-type cultivars (Bosug, Pungsannamul, Taekwang and Hutcheson) were evaluated to determine forage yield and quality at stage R6 in 2010. Hutcheson had the highest forage yield with 24.7 t/ha in fresh weight (FW) and 6.6 t/ha in dry matter (DM) among cultivated grain soybeans. A selected line W11 had highest FW (25.7 t/ha) and DM (6.2 t/ha) among G. max × G. soja selections. Five selected lines (W02, W06, W11, W13 and W18) had similar forage yield compared to Hutcheson. Generally the 25 selected lines derived from G. soja × G. max had thinner main stems and branches which lead to get more edible forage than cultivated soybeans. When selected lines were evaluated for their feed quality as per forage grade by American Forage and Grassland Council, 23 lines had a crude protein with more than 19%; 9 lines had a neutral detergent fiber below 40%; 23 lines had an acid detergent fiber below 31%; 24 lines had a digestible dry matter of more than 65%; 13 lines had a dry matter intake of more than 3.0%, and 17 lines had an relative feed value higher than 151 or were equivalent to prime grade. And all of 25 inbred lines were equivalent to Grade 1. Therefore, inter-specific cross between G. max and G. soja will be an excellent way to develop forage soybean with good yield and quality.
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This study was conducted to evaluate forage yield and quality for the accessions derived from inter-specific cross between wild and cultivated soybeans. First of all, three soybean lines (W02, W09 and W11) derived from PI483463 (Glycine soja) x Hutcheson (G. max) and three cultivated soybeans (Daewon, Pungsannamul and Bosug) were evaluated to determine forage yield and quality at the R2 (full bloom stage), R4 (full pod stage) and R6 (full seed stage) stages of development. Based on forage yield and quality, R6 was determined the optimal harvest stage to provide forage of high quality and adequate quantity for animals in both lines derived from G. soja × G. max and cultivated soybean.

Second, 25 selected lines from PI483463 × Hutcheson or S-100 (G. max) × PI483463 and four common seed-type cultivars (Bosug, Pungsannamul, Taekwang and Hutcheson) were evaluated to determine forage yield and quality at stage R6 in 2010. Hutcheson had the highest forage yield with 24.7 t/ha in fresh weight (FW) and 6.6 t/ha in dry matter (DM) among cultivated grain soybeans. A selected line W11 had highest FW (25.7 t/ha) and DM (6.2 t/ha) among G. max × G. soja selections. Five selected lines (W02, W06, W11, W13 and W18) had similar forage yield compared to Hutcheson. Generally the 25 selected lines derived from G. soja × G. max had thinner main stems and branches which lead to get more edible forage than cultivated soybeans. When selected lines were evaluated for their feed quality as per forage grade by American Forage and Grassland Council, 23 lines had a crude protein with more than 19%; 9 lines had a neutral detergent fiber below 40%; 23 lines had an acid detergent fiber below 31%; 24 lines had a digestible dry matter of more than 65%; 13 lines had a dry matter intake of more than 3.0%, and 17 lines had an relative feed value higher than 151 or were equivalent to prime grade. And all of 25 inbred lines were equivalent to Grade 1. Therefore, inter-specific cross between G. max and G. soja will be an excellent way to develop forage soybean with good yield and quality.

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약배양 이용 벼멸구, 흰잎마름병 및 줄무늬잎마름병 저항성 복합 내병충성 벼 계통 육성
Development of Multi-resistant Lines to Brown Planthopper, Bacterial Blight, and Rice Stripe Virus using Anther Culture in Rice
Hyun-Su Park, So-Hyeon Baek, Woo-Jae Kim, Ji-Ung Jeung, Jong-Hee Lee, Ki-Yong Ha, Jong-Ho Park, Jeong-Kwon Nam, Man-Kee Baek, Jae-Soo Yoo, Chae-Hoon Paik, Tae-Hwan Noh, Ki-Young Kim, Young-Chan Cho, Bo-Kyeong Kim, Jeom-Ho Lee
Korean. J. Breed. Sci. 2014;46(1):78-89.   Published online March 31, 2014
DOI: https://doi.org/10.9787/KJBS.2014.46.1.078

This study was conducted to develop multi-resistant lines to brown planthopper, bacterial blight, and rice stripe virus using anther culture in rice. A total of 213 double haploid lines were developed the cross between HR26234-12-1-1 conferring resistant to bacterial blight and rice stripe virus and SR30071-3-7-23-6-2-1-1 conferring resistant to brown planthopper, bacterial bight, and rice stripe virus. Using DNA molecular marker, HR26234 and SR30071 were confirmed to have Xa3+xa5+Stvb-i and Bph18+Xa4+Stvb-i, respectively. All double haploid lines carried Stvb-i, and Bph18+Xa3, Bph18+Xa4, Bph18+Xa3+xa5, Bph18+Xa4+xa5, bph18+Xa3, bph18+Xa4, bph18+Xa3+xa5, and bph18+Xa4+xa5 combinations were identified. Segregation distortions such as no combinations carrying Bph18(or bph18)+xa5+Stvb-i and fewer lines carrying Bph18 than bph18 were occurred in DH population. Brown planthopper resistant lines carrying Bph18 showed longer culm length than susceptible lines. Selected Bph18+Xa4+xa5+Stvb-i combination lines with short culm conferred resistant to brown planthopper, bacterial blight, and rice stripe virus, while showed deleterious effects such as spikelet sterility, lower yield, and vulnerable to lodging than standard and comparative varieties. Using anther culture, we rapidly developed multi-resistant lines to brown planthopper, bacterial blight, and rice stripe virus. However, distorted segregation in DH population and linkage drag with Bph18 were obstacles to develop practical multi-resistant cultivars.

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두부전용 이모작 안전 다수성 콩 신품종 ‘만풍’
A New Tofu Soybean Cultivar ‘Manpoong’ with High Yield for Double Cropping System
Eun-Seob Yi, Jong-Hyong Lee, Byeng-Yul Choi, Yeong-Soo Lee, Seng-Ki Kim, Hee-Dong Kim, Hong-Tae Yun
Korean. J. Breed. Sci. 2014;46(1):90-95.   Published online March 31, 2014
DOI: https://doi.org/10.9787/KJBS.2014.46.1.090

A new soybean cultivar ‘Manpoong’ for tofu soybean was released by ARES, Gyeunggi Province in 2009. The goals of breeding were for large seed size, high yield, and resistance to disease such as bacterial pustule. ‘Manpoong’ was derived from the cross between ‘Dongsan 121’, which has large seed size, yellow seed coat, and characteristics of superior tofu, and ‘Sprite 87’, which has early maturity. The preliminary, advanced and regional yield trials for evaluation and selection were carried out in 2003 and from 2005 to 2009, respectively. ‘Manpoong’ has a determinate growth habit with white flower, grey pubescence, dark brown pod color, yellow seed coat, yellow cotyledon, elongated seed shape, and large seed size(29.5 g per 100 seeds). The maturing date was Sept. 29. The content of crude protein, crude oil, unsat. fatty acids, isoflavone, total amino acid which affected the production rate of tofu was higher than those of the check cultivar ‘Taekwangkong’. The production rate of tofu was 15 percent higher than the check cultivar ‘Taekwangkong’. The average yield of ‘Manpoong’ was 2.87 MT/ha in the regional yield trials(RYT) carried out in two location at double cropping system in Korea from 2007 to 2009, which was 12 percent higher than the check cultivar ‘Taekwangkong’.

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중생 최고품질 내병 내도복 다수성 신품종 벼 품종 ‘대보(大寶)’
A High Qualilty Rice Variety with Lodging Tolerance, ‘Daebo’
Jeong-Il Kim, No-Bong Park, Ji-Yoon Lee, Oh-Deog Kwon, Dong-Soo Park, Jae-Ki Chang, Un-Sang Yeo, Jung-Hun Kang, Jong-Hee Lee, Jun-Hyun Cho, You-Cheon Song, Ki-Chang Jang, Kyung-Jin Choi, Seong-Hwan Oh, Un-Ha Hwang, Woo-Jae Kim, Seong-Tae Park, Hang-Weon Kang, Jong-Ki Lee
Korean. J. Breed. Sci. 2014;46(1):96-102.   Published online March 31, 2014
DOI: https://doi.org/10.9787/KJBS.2014.46.1.096

A rice variety ‘Daebo’ is a japonica rice (Oryza sativa L.) with good eating quality, lodging tolerance, and resistance to rice stripe virus (RSV) and blight bacterial disease (BB). It is developed by the rice breeding team of Yeongdeog Substation, National Institute Crop Science, RDA in 2011. This variety derived from a cross between ‘YR21247-68-1’ with good plant type and ‘Yeongdeog35’ with good eating quality conducted in 2002 summer season. A promising line, YR23940-B-17-1-2, selected by bulk and pedigree breeding method was designated as the name of ‘Yeongdeog51’ in 2008. After the local adaptability test was carried out at seven locations from 2009 to 2011, ‘Yeongdeog51’ was released as the name of ‘Daebo’ in 2011. ‘Daebo’ is short culm length as 63 cm and medium-growth duration. This variety is resistant to races, K1, K2, and K3 of bacterial blight and stripe virus and moderately resistant to leaf blast disease resistance. ‘Daebo’ has translucent and clear milled rice kernel without white core and belly rice, and good eating quality as a result of panel test. The yield potential of ‘Daebo’ in milled rice is about 5.93 MT/ha at ordinary fertilizer level of local adaptability test. This cultivar would be adaptable to middle plain, south plain, mid-west costal area, and south mid-mountainous area.

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내도복 저아밀로스 벼 신품종 ‘진상’
A New Variety ‘Jinsang’ with Lodging Tolerant and Low Amylose Content
Woo Ju Hong, Min-Young Yoon, Won-Hee Ra, Jae-Wan Park, Sun-Kyung Min, Soon-Wook Kwon, Yoo-Hyun Cho, Yong-Jin Park
Korean. J. Breed. Sci. 2014;46(1):103-107.   Published online March 31, 2014
DOI: https://doi.org/10.9787/KJBS.2014.46.1.103

‘Jinsang’ is a new japonica rice variety was derived from a cross between ‘Yumetsukushi’ as a high grain quality and ‘Milky queen’ with low amylose content and good eating quality in 2000, and selected by pedigree breeding method until M8 generation. As a result, a promising line, JCH33M-15-1-9-1-5-1-1-1, was advanced and designated as the name of ‘HJ-2’ in 2009. This variety has about 111 days to heading after sowing and has 75.2 cm culm height. Milled rice of that shows dull and medium short grain and 1,000-grain weight was about 20.9 g. It has low amylose content of 11.9% and lower protein content of 6.8%. The yield potential of ‘Jinsang’ in brown rice was about 4.57 MT/ha by ordinary culture in replicated yield trial test.

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