Korean Journal of Breeding Science

"Seon Suk Kim"

Articles

오프리× 금강 RIL 집단에서 알레르기 저감 및 제빵 품질 관련 유전자형의 분자표지 선발 Molecular Marker Selection of Genotypes Associated with Reduced Allergenicity and Bread-making Quality in an Ofree ×Keum gang Recombinant Inbred Line Population: Seon Suk Kim, Sumin Hong, Jae-Han Son, Chul Soo Park; Korean. J. Breed. Sci. 2026;58(3):263-273. Published online September 1, 2026; DOI: https://doi.org/10.9787/KJBS.2026.58.3.263

Abstract
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An important objective in wheat breeding is the development of cultivars with reduced allergenicity and enhanced bread-making quality. In this study, we conducted molecular marker analysis using 1,780 F₁₀ recombinant inbred lines derived from a cross between the hypo-allergenic cultivar Ofree and the Korean bread wheat cultivar Keumgang, and assessed the genotypes associated with ω-5 gliadin, α-gliadin epitopes, bread-making quality, grain hardness, total gliadin content, and the glutenin/gliadin ratio. A Gli-B1 deletion-based ω-5 gliadin-deficient genotype was detected in 18% of the population, whereas no lines lacking Gli-D1-derived ω-5 gliadin or α-gliadin epitopes were identified. The AX-108761918 marker was found to have segregated into TT and GG genotypes at frequencies of 48% and 52%, respectively. Eleven lines carrying favorable combinations of Glu-D1d, Pinb-D1b, and TaIAA10-6D Hap II were selected as promising bread wheat candidates, and a further nine lines combining the Gli-B1 deletion with Glu-D1d and Pinb-D1b were identified as candidates with both reduced-allergenicity and enhanced bread-making quality genotypes. SDS-PAGE analysis confirmed patterns of storage proteins consistent with the results obtained for marker genotyping. These selected lines may provide valuable breeding material for the development hypo-allergenic wheat cultivars with enhanced bread-making quality in Korea.

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국내 밀 유전자원 주요 형질 유전자 변이 분석과 군집특성 비교 Genotypic Variation and Phenotypic Clustering of 515 Korean Wheat Germplasm Based on Agronomic and Grain Traits: Seon Suk Kim, Sumin Hong, Myoung-Goo Choi, Chang-Hyun Choi, Chon-Sik Kang, Kyeong-Min Kim, Chul Soo Park; Korean. J. Breed. Sci. 2025;57(3):231-249.
Published online September 1, 2025; DOI: https://doi.org/10.9787/KJBS.2025.57.3.231

Abstract
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A comprehensive evaluation of 515 Korean wheat germplasms, including cultivars, experimental lines, and landraces, was conducted over 2 years under upland field conditions to characterize major agronomic and grain traits. Allelic variation at 13 key functional loci was assessed using Kompetitive Allele-Specific PCR (KASP) and PCR-based markers. The winter-type vrn-A1 with a single copy (CNV=1; 40.2%) advanced heading by approximately 5 days compared to multiple-copy genotypes, and winter-type vrn-B1 (88.5%) advanced heading by 2 days compared to the spring-type. The photoperiod-insensitive alleles Ppd-B1a (5.6%) and Ppd-D1a (76.3%) advanced heading by 3 and 4 days, respectively, with a combined effect of up to 6 days. Semi-dwarfing alleles Rht-B1b and Rht-D1b showed reduced culm lengths of 2.1 cm and 4.7 cm, respectively, and the Rht-B1a/Rht-D1b genotype was 6 cm shorter than Rht-B1a/Rht-D1a. The Pina-D1a/Pinb-D1a genotype had the lowest kernel hardness value (32.2), whereas Pina-D1b/Pinb-D1a had the highest (60.5). The thousand kernel weight ranged from 36.1 mg to 42.5 mg depending on the allelic combinations of TaCwi-A1, TaGW2-6A, and TaSus2-2B. Cultivars and experimental lines were clearly distinguished from landraces based on phenotype-based clustering, with the majority of cultivars (81.6%) and experimental lines (68.3%) grouped into cluster III. In contrast, landraces were predominantly distributed in clusters I (55.1%) and II (29.2%). Random forest analysis identified four genes, Ppd-D1, Pina-D1, Pinb-D1, and WAPO-A1, as major contributors to cluster classification. Cluster III was highly enriched with alleles favorable for earliness (Ppd-D1a, 98.3%) and grain hardness (Pina-D1b and Pinb-D1b, 57.9%). WAPO-A1b, an allele associated with an increased spikelet number per spike, was more frequently observed in clusters I (94.6%) and II (79.1%) than in cluster III (58.4%).

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GWAS identifies major loci for agronomic and yield-related traits in wheat through cross-population validation
Sumin Hong, Jae-Han Son, Chon-Sik Kang, Changhyun Choi, Youngjun Mo, Jae Yoon Kim, Dae Yeon Kim, Kyeong-Min Kim, Chul Soo Park
Journal of Integrative Agriculture.2026;[Epub] CrossRef
Inter‐annual and regional variability of agronomic and grain quality traits of major Korean wheat cultivars under multi‐environmental conditions
Goeun Lee, Jinhee Park, Kyeong‐Min Kim, Chon‐Sik Kang, Sookjin Kim, Jeong‐Heui Lee, Hyun‐jin Jung
Journal of the Science of Food and Agriculture.2026; 106(12): 7512. CrossRef