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Volume 53(2); June 2021

Articles

국내산 밀 전분 및 종실 경도 특성이 생면 품질에 미치는 영향
Effect of Starch and Hardness Characteristics of Korean Wheat Cultivars on Noodle Quality
Jin Hee Park, Chul Soo Park, Chon-Sik Kang, Jinwoo Yang, Han-Yong Jung, Kyeong-Min Kim, Chang-Hyun Choi, Jae-Han Son, Jiyoung Son, Tae-Il Park, Kyeong-Hoon Kim
Korean. J. Breed. Sci. 2021;53(2):83-95.
Published online June 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.2.83

This study was conducted to investigate the effect of starch properties on the texture of cooked noodles from Korean wheat. The genetic composition of GBSS I (granule bound starch synthase I, called waxy protein) and puroindoline, which affect the amylose content and kernel hardness, was also evaluated. Waxy wheats carrying Wx-1 null alleles showed clearly different starch properties, high swelling power, pasting viscosity, breakdown and paste clarity, unsuitable texture of cooked noodles, and low hardness and springiness. Two partial waxy soft wheats carrying single or double null alleles at the Wx-1 locus gene, and Pina-D1a and Pinb-D1a alleles exhibited a softer and higher elasticity texture of the cooked noodles than Korean wheats carrying wild-type Wx-1 null alleles. There were no significant differences in the starch properties and texture of cooked noodles according to the puroindoline composition. A principal component analysis showed a strong negative relationship between the amylose content and starch swelling power, and these traits also improved the springiness and cohesiveness of the cooked noodles prepared from non-waxy Korean wheat. Joongmo2012, a double null partial waxy wheat, showed higher starch swelling power and springiness of the cooked noodles than other non-waxy Korean wheats.

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The color and functionality of paprika fruits (Capsicum annuum L.) are important factors that determine consumption preferences. Paprika contains a large amount of functional phytochemicals, such as carotenoids and polyphenols. In this study, the total polyphenol content by total phenol content (TPC) assay, antioxidant activity by 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferric reducing antioxidant power (FRAP), and total red pigment content by American spice trade association (ASTA) color were evaluated in a total of 93 commercial cultivars and germplasms of red paprika. In results, the total polyphenol content, antioxidant activity by ABTS and FRAP, and total red pigment content ranged from 6.35-11.84 mg GAE⋅g-1 dry weight (DW), 19.80-44.84 mg trolox⋅g-1 DW, 8.82-31.06 mg trolox⋅g-1 DW, and 5.30-75.03 ASTA value, respectively. For each trait, there were 2, 31, 16, and 44 germplasms, respectively, which were higher than those of the commercial cultivars. The correlation analysis between the total polyphenol content and antioxidant activity (ABTS and FRAP) showed highly positive correlations, with r=0.69 and r=0.75, respectively. In contrast, the ASTA values showed very low negative correlations with ABTS (r=-0.15) and FRAP (r=-0.26). These results imply that the antioxidant activity in paprika was largely affected by the total polyphenol content but was hardly affected by the ASTA value. Accessions 30, 31, and 56 were selected with high red pigment content, and accessions 5, 21, 24, and 26 had high antioxidant activity as well as high polyphenol content. These germplasms will be useful for the development of new paprika varieties with high red pigment content or high antioxidant activity.

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Integrating RNA Sequencing and Quantitative Trait Locus Mapping to Identify Potential Candidate Genes for Flooding Tolerance in Soybean
RNA 시퀀싱과 QTL 분석을 통한 콩 내습성 관련 후보유전자 탐색
Sanjeev Kumar Dhungana, Jeong-Hyun Seo, Ji-Hee Park, Jung-Sook Sung, Hong-Sik Kim, Beom-Kyu Kang, Sang-Ouk Shin, In-Youl Baek, Chan-Sik Jung
Korean. J. Breed. Sci. 2021;53(2):105-115.
Published online June 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.2.105

Flooding stress causes a significant reduction in soybean yield. The development of flood-tolerant cultivars is an effective way to minimize yield loss due to flooding. Information on candidate genes for flooding tolerance is useful for developing tolerant lines. The objective of this study was to identify potential candidate genes for flooding tolerance in soybean by integrating the results of a quantitative trait locus analysis and RNA sequencing. A total of 19 genes showed good amplification in capillary electrophoresis and were further analyzed through a reverse transcription quantitative polymerase chain reaction (qRT-PCR); two of these genes showed differential expression among tolerant and susceptible lines. The expression of Glyma.12g030900 and Glyma.10g050300 in leaf and root tissues, respectively, was higher in several tolerant lines than in the susceptible lines under flooding stress. The chlorophyll index of the tolerant lines was also consistently higher than that of the susceptible lines over two years, supporting the qRT-PCR results. This study provides useful information on flooding tolerance in soybeans.

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The bulb onion (Allium cepa L.), one of the most important vegetables worldwide, contains various functional compounds such as quercetin, allicin, and flavonoids. Red onions are rich in anthocyanins, a flavonoid that is a functional phytochemical with antioxidative and anticancer activities. In the previous study, two quantitative trait loci (QTLs) (qAC4.1 and qAC4.2) controlling the anthocyanin content were identified on chromosome 4 in an F2 population derived from a cross between A. cepa ‘SP3B’ and ‘H6’. In this study, we developed single nucleotide polymorphism-based high-resolution melting (HRM) markers linked to QTLs qAC4.1 and qAC4.2. In addition, we constructed a new genetic linkage map of chromosome 4 using HRM markers and performed a QTL analysis. The QTL qAC4.1 was false, while qAC4.2 was a major QTL. The QTL peak position, logarithm of the odds value, and phenotypic variance explained of qAC4.2 was 53.6 cM, 7.45, and 22.51%, respectively. Four HRM markers (AC4.2_65336.1_1123-HRM, AC4.2_53230.3_454-HRM, AC4.2_11999.1_756-HRM, and AC4.2_14596.1_345-HRM) within the QTL region of qAC4.2 were developed in this study. The average anthocyanin content of B (homozygous paternal) genotypes was higher than that of A (homozygous maternal) and H (heterozygous) genotypes for all markers. Consequently, these markers will be useful for marker-assisted selection to develop onion cultivars with high anthocyanin content.

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Understanding plant morphological and seed characteristics is an essential step for the utilization of genetic resources to improve targeted traits in soybean breeding programs. The purpose of this study was to evaluate the agronomic traits and seed pigments of 469 black soybean germplasms with green cotyledons to identify useful resources using multivariate analyses, correlation, principal component analysis (PCA), and cluster analyses. The results showed that the range of flowering days from planting were 37.7-71.5 days, harvesting days from planting were 112.3-153.0 days, plant height was 49.6-151.6 cm, and 100-seed weight was 9.1-49.3 g. According to the correlation analyses with agronomic traits, flowering and harvesting days were positively correlated with the plant height, number of nodes, and number of branches. Additionally, it was found that the chlorophyll a and total chlorophyll content had the greatest effect on the green color of the cotyledon. Based on the PCA, 13 quantitative traits were compressed into three components. The first three principal components accounted for 82.5% of the total variance. In this study, black soybean germplasms with green cotyledons were divided into four clusters by the K-means cluster analysis (non-hierarchical procedure), based on the PCA. The cluster analysis showed that chlorophyll a and plant height had a significant effect on the clustering of the black soybean germplasms used in this study. This study provides opportunities to easily select useful genetic resources for the development of new black soybean cultivars.

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New Cultivar Developed
열대 적응형 조숙 다수성 단교잡종 옥수수 ‘케이엠2’
KM2: a Tropical Single Cross Corn Hybrid with Early Mature and High Grain Yield
Hwan-Hee Bae, Seong-hyu Shin, Young-Sam Go, Jun Young Ha, Yoo-Chan Choi, Jae-Han Son, Tae-Wook Jung, Seong-Bum Baek, Jung-Tae Kim, Jin-Seok Lee, Beom-young Son, Ki-Young Kim
Korean. J. Breed. Sci. 2021;53(2):140-144.
Published online June 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.2.140

‘KM2’, a maize hybrid grain was developed for the Southeast Asian seed market by the maize breeding team of the Rural Development Administration (RDA), South Korea 2018. ‘KM2’ was bred by crossing two inbred lines, ‘15VL065’ and ‘KS155’. After a advanced yield trial at Dong Trieu, Vietnam, for the first cropping cycle in 2016, country adaptability trials were performed twice in three different countries: Vietnam, Cambodia, and Indonesia, in 2016 and 2017. ‘KM2’, a yellow intermediate seed for food and feed, adapts well to the northern Vietnam, northwestern Cambodia, and Central Java in Indonesia. It is an early maturing high-grain yield F1 hybrid. This hybrid is resistant to downy mildew, but not corn rust. The silking date of ‘KM2’ is 10 days earlier than that of ‘LVN10’, a leading cultivar in Vietnam. ‘KM2’ produced grain yields of 811 kg/10a, 845 kg/10a, and 783 kg/10a in Vietnam, Cambodia, and Indonesia, respectively, showing even higher grain yield than the countries’ respective leading cultivar. ‘KM2’ produced 61%, 17%, and 14% higher grain yield in Vietnam, Cambodia, and Indonesia, respectively, than their respective control cultivars (‘LVN10’, ‘CP888’, ‘P21’) (Registration No. 8192).

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백립계 대립 붉은곰팡이병 중도저항성 제빵용 밀( L.) ‘백강’
A Wheat Cultivar ‘Baekkang’ with Good Bread Quality, White Grain Wheat, Large Kernel and Moderate Resistance to Fusarium Head Blight
Kyeong-Min Kim, Kyeong-Hoon Kim, Chang-Hyun Choi, Han Young Jeong, Jinhee Park, Sun-Mok Yang, Jiyoung Shon, Tae-Il Park, Chon-Sik Kang
Korean. J. Breed. Sci. 2021;53(2):145-153.
Published online June 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.2.145

A new winter wheat (Triticum aestivum L.) cultivar ‘Baekkang’ was developed by the National Institute of Crop Science (NICS) and Rular Development Administration (RDA) in 2015. Its heading date was April 23, and its maturity date was June 3, similar to that of ‘Keumkang’. ‘Baekkang’ had a shorter culm length (75 cm), longer spike length (8.0 cm), more spikes per m2 (703), and more 1,000-grain weight (47.5 g) than those of ‘Keumkang’, which were 76 cm, 7.6 cm, 631, 46.4 g, respectively. ‘Baekkang’ was not a winter hardy crop and is susceptible to powdery mildew. However, it has moderate resistance to fusarium head blight. The average grain yield in the advanced yield trial was 5.5 MT/ha, 20% more than ‘Keumkang’. In the regional yield trial, this average yield was 5.1 MT/ha upland and 5.2 MT/ha in the paddy field, which were 10% and 18% more than that of ‘Keumkang’, respectively. Baekkang’s flour yield (71.2%) and flour lightness (92.40) showed similarities to those of ‘Jokyung’. ‘Baekkang’ also showed a higher protein content (12.4%), gluten content (10.1%), and SDS-sedimentation volume (60.0 ml). These results showed that the ‘Baekkang’ flour’s dough strength was greater than that of ‘Jokyung’. Baekkang’s high molecular weight gluten subunits composition was Glu-D1 (5+10), granule-bound starch synthase composition was Wx-A1 (a), Wx-B1 (a), and Wx-D1 (a), and puroindoline composition was Pina-D1(a) and Pinb-D1(b) (Registration No. 6966).

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이모작 적응 조생 복합내병충성 고 바이오매스 사료용 벼 ‘조우’
‘Jowoo’, Early Maturing, Multiple Disease and Insect Resistant High Biomass Yielding Rice Cultivar for Winter Forage Crop-Whole Crop Silage Rice Double Cropping
Eok-Keun Ahn, Yong-Jae Won, Jeom-Ho Lee, Eung-Gi Jeong, Hyang-Mi Park, Ha-Cheol Hong, Jae-Ki Chang, Ung-Jo Hyun, Kuk-Hyun Jung, Jeong-Heui Lee
Korean. J. Breed. Sci. 2021;53(2):154-162.
Published online June 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.2.154

‘Jowoo’, an early maturing, high biomass yielding rice (Oryza sativa L.) cultivar with high feed value and multiple disease-insect resistance, was developed for winter forage crop-whole crop silage (WCS) rice double cropping. It was derived from a cross between a high biomass and multiple disease resistant line ‘Suweon519’ and an early flowering, high yielding Tong-il type variety ‘Gaya’ that harbored Bph3 and BPH26, conferring brown planthopper (Bph) broad resistance. This cultivar had a growth period of approximately 106 days from seeding to heading, 97 cm culm length, 24 cm panicle length, 12 panicles per hill, 120 spikelets per panicle, and 1,000-grain weight of 22.1 g as brown rice in the central plain region of Suwon. This early flowering rice variety was tolerant to premature germination and cold stresses, especially showed higher grain fertility than ‘Nokyang’ at a cold-water (17℃) irrigated screening nursery. Further, ‘Jowoo’ exhibited high lodging resistance in the yellowing ripening stage, an optimal period for harvest. In addition, ‘Jowoo’ was resistant to leaf and neck blast, bacterial blight (race K1, K2, K3, K3a), rice stripe virus, and small Bph and was also moderately resistant to Bph. Its average dry matter yield for three years reached 18.2 MT/ha, the highest among early flowering rice varieties and 19% higher than that of ‘Nokyang’. This cultivar had 72.2% total digestible nutrients, which is also higher than that of ‘Nokyang’. In the Korean peninsula, ‘Jowoo’ grows well in the central and southern plains and can be harvested 30 days after heading. This helps improve its feeding value and digestion rate in livestock (Grant No. 8133).

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수요자 참여형으로 육성된 최고품질 벼 ‘알찬미’
‘Alchanmi’ - A High-Quality Rice Bred with Stakeholder Participatory Program
Ung-Jo Hyun, Yong-Jae Won, Eung-Gi Jeong, Eok-Keun Ahn, Jeong-Heui Lee, Kuk-Hyun Jung, Hyang-Mi Park, Yong-Hee Jeon, Jieun Kwak, Nak-Sig Sung, Jeong-Cheon Kim, Hyeon-Suk Jeong, Dong-Yun Shin, Jae-Woo Suk
Korean. J. Breed. Sci. 2021;53(2):163-169.
Published online June 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.2.163

‘Alchanmi’, a japonica rice variety, was developed from a cross between ‘Junam’ and ‘Chilbo’ by a rice breeding team at the National Institute of Crop Science and Icheon, Agricultural Technology Center in 2018. The heading date of ‘Alchanmi’ was August 10 in the middle plain area—two days later than that of ‘Hwaseong’. ‘Alchanmi’ had a culm length of 69 cm, 16 cm shorter than that of ‘Hwaseong’, and 103 spikelets per panicle. The viviparous germination rate of ‘Alchanmi’ was 24.4%. It showed resistance to blast, bacterial blight (K1, K2, K3 race), and stripe virus, but was susceptible to the K3a race of bacterial blight, dwarf and black streak dwarf viruses, and plant hoppers. The milled rice of this variety exhibited a translucent and medium short grain shape. The cooked rice grains of ‘Alchanmi’ had an excellent palatability index (0.7) and showed lower amylose content (18.6%) than that of ‘Hwaseong’. The grain milling characteristics of ‘Alchanmi’ were better than those of ‘Hwaseong’, especially the head rice milling recovery ratio and head rice ratio (96.2%). ‘Alchanmi’ showed 5.83 MT/ha of milled rice productivity at 6 sites under ordinary cultivation (Registration No. 8132).

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밥맛이 우수한 경기지역 적응 조생종 벼 ‘정드림’
‘Jungdream’—An Early Maturing and Good Eating-Quality Rice Cultivar Suitable for Growing in the Gyeonggido Province, South Korea
Jung-Hee Jang, Byoung-Rourl Choi, Tae-Jin Won, Ji-Hye Lee, Jong-Hyeong Lee, Sang-Wook Han, Jae-Eun Jang, Jae-Soon Seo, Jung-Hyeun Ji, In-Tae Park, Hae-Chun Choi, Gwang-Ho Kim
Korean. J. Breed. Sci. 2021;53(2):170-176.
Published online June 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.2.170

‘Jungdream’ is an early maturing, high-quality rice cultivar developed in 2017 by the crop breeding team at Crop Research Division, GARES, Hwaseong, Korea. ‘Jungdream’ was first developed in 2008 from crossing a medium-late maturity cultivar ‘Chilbo’ and an early maturity cultivar ‘Ungwang’. The heading date of this cultivar is July 28th in the Gyeonggido Province. Its culm length is 63 cm, and it has 19 panicles per hill and 82 spikelets per panicle. It is a medium-small grain brown rice cultivar with a 1000-grain weight of 20.6 g. Its cold tolerance is lower than that of ‘Hwaseong’, which is the standard cultivar in the central region, as is its degree of lodging tolerance. ‘Jungdream’ is resistant to rice blast and stripe virus diseases but is susceptible to bacterial leaf blight and other viral diseases and pests. The appearance of milled ‘Jungdream’ rice is clear, and its amylose content is 16.5%, which is lower than that of ‘Hwaseong’. The palatability of cooked ‘Jungdream’ rice is better than that of ‘Chucheong’, which is the main cultivated crop in Gyeonggido. Its milling ratio is 75.0%, which is lower than that of ‘Hwaseong’. The yield of milled ‘Jungdream’ rice was 5.42 MT/ha in the local adaptability tests during standard cultivation in four regions from 2015 to 2017. ‘Jungdream’ is highly adaptable to the environmental conditions in the Gyeonggido Province (Registration No. 8236).

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고품질 중간찰 향미벼 ‘골든퀸 3호’
Golden queen 3: A High-quality Rice Variety with Low Amylose Contents and Aroma
Seong-Gyu Jang, Wondo Lee, Yong-Jin Park, Young-Sang Lee, Joohyun Lee, Jeonghwan Seo, Yoo-Hyun Cho, Soon-Wook Kwon
Korean. J. Breed. Sci. 2021;53(2):177-186.
Published online June 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.2.177

‘Golden queen 3’, an aromatic rice variety, was developed by Seedpia inc. (Suwon, Korea) breeding company in 2014. This cultivar was derived from a cross between ‘HJ-11’, a high-yield, aromatic line, and ‘JCH33M-15-1-9-1’, which has a low amylose content. During the F3-F8 generation, breeding lines were selected using a pedigree breeding method. The most promising line (JS9-3-73-15-5-1-5-3-1-1) was advanced and named ‘SP005’ in 2013. This variety was headed on August 13, two days later than ‘Hwayeong’ in the ‘in middle plane’ is commonly used to distinguish rice cultivation areas in Korea, so I think it's no problem to the understanding for readers (including farmers). The culm and panicle lengths of this variety were 75.7 cm and 20.1 cm, respectively. It has approximately 14.7 tillers per hill and 104.8 spikelets per panicle, and the 1000-grain weight was 21.8 g. The yield performance was 5.47 MT/ha in the local adaptability test, and the amylose content of this variety was 12.5%. 2-Acetyl-1-pyrroline was detected as the major volatile ingredient in aromatic rice, and a 1 bp insertion on the 14th exon (badh2.7 allele) was identified in the Badh2 gene. (Registration No. 7308).

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대과종 단감 신품종 ‘단누리’
A New Sweet Persimmon Cultivar, ‘Dannuri’, with Large-Sized Fruits
Eun-Gyeong Kim, Yeo-Ok Park, Ji-Young Son, Gwang-Hwan Ahn, Hae-Suk Yoon, Young-Ho Chang
Korean. J. Breed. Sci. 2021;53(2):186-189.
Published online June 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.2.186

To develop early ripening sweet persimmon varieties with high fruit qualities, ‘Danyeon 104’ was crossed with ‘Taishuu’ at the Sweet Persimmon Research Institute, Gyeongsangnam-do Agricultural Research & Extension Services in 2010. In 2015, ‘10-1-60’ was selected and investigated from among the seedlings of this cross, and in 2018, it was confirmed as a superior variety for the production of large-sized sweet persimmon with good fruit qualities and was named ‘Dannuri’. The ‘Dannuri’ trees grew vigorously in the early stage and exhibited a spreading shape as they aged. The flowers possessed both the female and male organs. The full blooming date of ‘Dannuri’ was May 22, which was close to that of ‘Taishuu’ (May 19). The average harvest time of ‘Dannuri’ was 143 days after the full bloom, and it matured approximately one week earlier than ‘Taishuu’. The fruits of ‘Dannuri’ were characterized by beautiful orange skin color with round flat shape with an average weight of 320 g—which was 1.2-fold higher than that of ‘Taishuu’ (265 g)—and soluble solids content of 18.4 °Brix, which was also higher than that of ‘Taishuu’ (17.2 °Brix). Additionally, the marketable fruit rate of ‘Dannuri’ was 90.0%, which was also higher than that of ‘Taishuu’ (70.2%), and this could be attributed to less skin blackening. For the stable production of ‘Dannuri’, it is necessary to maintain proper tree vigor by paying extra attention to the use of organic compost (Registration No. 8504).

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