1전북대학교 농업생명과학대학 원예학과
2농촌진흥청 국립원예특작과학원 과수과
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Copyright © 2022 by the Korean Society of Breeding Science
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Summary of SNP detection and HRM primer design between grape cultivars ‘Tano Red’ and ‘Ruby Seedless’.
| Segregation type | Number of SNP markers | Number of HRM primers designed (A) | Number of polymorphic HRM primers (B) | Success rate (%) (B/A*100) | ||
|---|---|---|---|---|---|---|
| ‘Tano Red’ | ‘Ruby Seedless’ | Symbol | ||||
| Homozygous | Heterozygous | nn×np | 853 | 449 | 251 | 55.9 |
| Heterozygous | Homozygous | lm×ll | 951 | 509 | 353 | 69.4 |
| Heterozygous | Heterozygous | hk×hk | 749 | 378 | 201 | 53.2 |
| Total | 2,553 | 1,336 | 805 | 60.3 | ||
List of HRM primer sets used in this study.
| Marker name | Chr. | Position (cM) | Forward primer | Reverse primer | SNP |
|---|---|---|---|---|---|
| C01_1119888-HRM | 1 | 0.0 | CCGCTTCACGTAGACTGAACTC | AGAATTGGAGTAAGTTGAGGTTGG | T/C |
| C01_1646914-HRM | 1 | 11.7 | GCCTATAAGCCTCATCAAGACTTC | AGGAAGGTTTCTCTCCCAATATCT | C/T |
| C01_3587166-HRM | 1 | 17.3 | GATATCTTCTCCATTCATTGCTGA | GGACGAAGATACCAAGAAGGTAGC | A/C |
| C01_3978740-HRM | 1 | 22.0 | AAACAGAAACACCATATGGGAACT | TATTTATTCATCATGCTTGCTGCT | A/C |
| C01_4119172-HRM | 1 | 24.6 | CAGCTTCGCACCTATGCTAACTAC | AGAGAAATGGAGTGAGAAACCCTA | C/A |
| C01_5578917-HRM | 1 | 30.3 | TAATGCCACTTAATTTGCAGGAAT | CTGAATCTTTCCAATGCTTCTGTT | C/T |
| C01_7367827-HRM | 1 | 32.6 | AAAAATGCATAAAATTGCAACTGA | CACCTATGCAAAACATTCACAGAG | A/C |
| C01_9408352-HRM | 1 | 38.9 | TGAACATGAGCTTTGCTAGGTTAC | GTGCTGTTGTAAGAAAGGAATGTG | C/T |
| C01_10642137-HRM | 1 | 47.4 | TACCAGAAGAGCAAAAGTCTTGG | CAAATGATTCCTTGTCAGTTTTTG | G/A |
| C01_11662715-HRM | 1 | 52.6 | AGTGAAGAAGGGTCGGTGATT | GTAGCTTTTGCATAGGGTTCCAT | C/T |
| C01_16586407-HRM | 1 | 57.3 | GGTTATTAAGAGGTGAAAGCCAAA | TGGAAATATTTAGTGCAGCAATTT | G/A |
| C01_12876858-HRM | 1 | 59.6 | ATGCCATATAGTGGAAGATTGGAT | GTGCACCATATGAAGAACCACTAA | A/G |
| C01_14656510-HRM | 1 | 60.5 | TTTATCAAACAGCCCAATGTCTTA | GTGCAGCTGAATCCTTACTTCTTT | C/T |
| C01_15212737-HRM | 1 | 61.6 | GCTCCCTCCCTCATTTACCTAT | TCACCAATCAAAAAGCTGAATAAA | G/A |
| C01_19514907-HRM | 1 | 67.1 | TGCTCAATGACGTATTTGAAAACT | GCAGCAACCAAACAACATTAGATA | G/A |
| C01_20364365-HRM | 1 | 70.8 | TCCTCTTTCTCATTCATTACAGCA | CTAGCAAGTTTGCTGAGGTTTGT | T/C |
| C01_20969991-HRM | 1 | 73.8 | ATTTCTAATGTGTAGCGCAAATGA | ATGGAGCCTTATATAATTGCCACT | T/C |
| C01_22138644-HRM | 1 | 77.6 | AACCACGTAGTTACAGACATGAGC | AATAGGAACTAGCGGGAAATAACC | G/T |
| C01_21845711-HRM | 1 | 81.7 | GATCTTAGATGCTGGATGCTTCTT | TATACCTTTTCCAGTCCATTTTCC | G/A |
| C01_23945977-HRM | 1 | 88.4 | TGAGCCTCCTTTAGTATCTGAAAA | ATCATGCAGCCAAAATAGTTCAAT | G/A |
| C02_525619-HRM | 2 | 0.0 | GATTGCTGCTCATCAATATCATC | ATGCTTCATTTTACTCATCCATTG | T/C |
| C02_235122-HRM | 2 | 6.5 | AAGAGGAGCTTTTGATGAAGTGAT | TCACTGCTAGAGTGTATTCATTGG | A/C |
| C02_1081253-HRM | 2 | 11.3 | ACCATATTCTGAGCAGAGTGAACA | TCCTGCAGAAAATTAAAAGTCAAA | C/T |
| C02_2209864-HRM | 2 | 18.6 | GAGTTCATGATGGCTGATTTCTTT | GGGACAACATTTAACCATAGAACC | A/G |
| C02_2365173-HRM | 2 | 24.3 | AGTTAGAACCTAACCTGAGCAGCA | TAATACTACAGCGGAAGGAAGGAG | A/G |
| C02_3529089-HRM | 2 | 29.9 | ACACTGCAAAACTCTTGAACTCAG | ATGCGTCTACAAAGTTGATGGAAC | G/T |
| C00_13513200-HRM | 2 | 38.0 | GTAGTAGTGGGCTTCCCGAGTTAT | CAGACCTGTAAAAGCAGCACAATA | A/G |
| C02_7880106-HRM | 2 | 55.4 | AACTGAAGCTGCGTATGATGTTC | CCTCTAAGATCAGATACGGTGGAG | T/G |
| C02_8030412-HRM | 2 | 56.4 | GGATTTCGATGCTCCAGGT | TCGTAATCGTAAATCACAATCTGC | G/A |
| C02_9303617-HRM | 2 | 58.1 | GCTCTTCTTCCAGAGTTATTTGTG | GGCTCTTGATTCTTCTTCAAGTTC | C/T |
| C02_8854992-HRM | 2 | 59.3 | TGAAGACATTCAATTTTGTTGGTT | TATGGTGTTTGTTTTAACGGAAGA | C/T |
| C02_8400378-HRM | 2 | 60.3 | AGCCTCATTGAAGACAATCTCATT | TCTTCTAATATGCTCTCTCCCCTAA | A/G |
| C02_10148394-HRM | 2 | 61.3 | AGAGGAATCAAATGGAGAGAATTG | GAAGTAAAGCAGCAACCACTGAT | C/T |
| C02_12868924-HRM | 2 | 61.6 | CAACAAAATCATTAGGTTTTTCAGTC | GCTAACAAAGAAATCACAAAAGCA | G/A |
| C02_18463767-HRM | 2 | 63.3 | ACGACGATTACATAGCTGAAACAC | TCATTCCCCTGTCATATAAAATGT | T/C |
| C02_10148217-HRM | 2 | 63.8 | ACAATTTTAATGGTTTTGGGTGTT | TCTGTGAATAATGAAAATGTAAATGAA | T/C |
| C02_8583872-HRM | 2 | 65.0 | AATTAAAAGGAAATGCAGCGAGT | CGGACCTTTTCGATCTCTTAAT | A/G |
| C02_17454742-HRM | 2 | 67.3 | CTTTGCTTCAGACTTTCTCACCTT | CAGAGAGTAAGCATAGTGCCAGAA | C/T |
| C02_17915790-HRM | 2 | 69.6 | CAACTCCATTTTGTCCAGCTC | GAAGGAGAGAATCACAGAGCAAGT | A/G |
| C03_46442-HRM | 3 | 0.0 | AAGAACAGGAACTTCCGAATATGA | GCTGCCCCTATGCATTATTTAAG | G/T |
| C03_3874067-HRM | 3 | 0.0 | AGAAAAAGCTGCTATTCATCCAAC | TACATGATCTTGCTCCTCTAACCA | C/T |
| C03_3253712-HRM | 3 | 2.2 | AACAGAACTCAAAGCAGGAGAACT | TCAAGAAGGCATACATTAGTTTCAA | C/T |
| C03_1613721-HRM | 3 | 4.3 | AGTATATGGACTTGGCTGAACAGG | TAGCAATATTGGAACTGTCCATCA | C/T |
| C03_2615665-HRM | 3 | 8.4 | AGTATCCTGAGATATGGGCTCTTG | ATGTGCAAGTCTACAAGCAACATC | A/G |
| C03_1541222-HRM | 3 | 9.0 | TTGCAGCAAAGGTATGAGATTACA | TCCATAACCCATAATCCAACTTCT | C/T |
| C03_5362712-HRM | 3 | 43.4 | CAAAGACGATGAACTAACTGGTCA | TGTTGCTAGAAAACCCTTTAAACC | T/C |
| C03_5799668-HRM | 3 | 48.0 | CTAATAGGAATTTTGCGAGGGAAG | TGTCTGGGTTCCTTCCATCTAGT | T/C |
| C03_18748644-HRM | 3 | 69.3 | GAAATATCAGCTGCCCTTTGTAAC | TATTCAACTTGGAAAACCACTCCT | T/G |
| C03_20276928-HRM | 3 | 72.8 | GAATACATTCTTTTCAGGGTGAAG | AATTGGGGTGTGTATACCAATTCT | C/A |
| C04_1252669-HRM | 4 | 0.0 | TCAAGTCCATATTGGATTTTCAGA | ATTTCTTCACACAGCGAAAGAAG | T/G |
| C04_1847860-HRM | 4 | 10.3 | TTAAATATGCCTGCTAACTGCTTG | AGTGATGAAGAATGAAGTACACTCAC | C/T |
| C04_2153062-HRM | 4 | 13.4 | ACATCATTCTCCAAGATACAGTGG | GAGAATGGGATGAGAATGAGACC | C/T |
| C04_5242968-HRM | 4 | 21.4 | ATCTGGATAAGCTAAGCAGCAATG | GGCAACAGAATATATCCAGGAAAA | T/G |
| C04_4199986-HRM | 4 | 25.2 | TTGATCAGTTTTAGTTGAGCTTCG | CTGGTTGCTGCCTAAATCTCTC | G/A |
| C04_4848299-HRM | 4 | 27.0 | CAGTACTTTTCTTCCACGTTTCCT | GTTCTGAAGGAGATTCGGAGTG | T/C |
| C04_7024950-HRM | 4 | 32.4 | TATGATGATGAAGTGAGGGAATTG | CAGCCTCGTAAACTACTTTCTCCT | G/A |
| C04_13595773-HRM | 4 | 37.8 | TAACTGATATTGCGAAAAACAGCA | ACAGGCACTTGATCAGTTGAAATA | T/C |
| C04_18374072-HRM | 4 | 51.7 | CTGCATTGAATGGAATTAAAATGG | ATACATTCTCTTGCTCGGGTTTAG | C/T |
| C04_19879714-HRM | 4 | 55.1 | AACCCACTTGCTCCAATCAC | AGTGCATTAGCTCCTACTCTAGGC | C/T |
| C00_24329427-HRM | 4 | 65.9 | AACTCAGTGCATTTCCTAGTGGAG | AAGAGAGCTTAGAAAGGGCTTACC | T/G |
| C04_21116712-HRM | 4 | 69.0 | ACAATTGCCATATGCTTTTCTCTT | TGGTTAAGATGTGCTTCATTGACT | A/G |
| C04_21518434-HRM | 4 | 72.8 | ATTGCAGCATTTTTATCAATTTCC | ATTTCACCATGAATGTCAAAAGAA | A/G |
| C04_21660082-HRM | 4 | 74.7 | ACCTCCTAACGATAGCAGCTTAGA | CGATATAGGAAATGTAAGGCCAGT | A/C |
| C04_22878658-HRM | 4 | 77.5 | GCTTGCTATCAATGCATTTTATTC | AGAAGACGACGACTTTCATGACTA | T/C |
| C04_23070555-HRM | 4 | 78.6 | CCAGGAAGTACAAATCGATGG | CGTTCGATCACGAGGTACTTG | G/A |
| C04_23556466-HRM | 4 | 79.6 | CAGCAGAGCATCCTGGTTCTA | CCAACACTAGCACACAAGACAGTT | T/C |
| C05_3279979-HRM | 5 | 0.0 | TTTTTAATTTCTCAATTCCGTTCC | GAAGGTGTATGACACTGCTGCTC | C/T |
| C05_4133797-HRM | 5 | 8.7 | GATGATGCTTATGATTGAGGACAC | TATACTTTCCAGTGGGAAACATGG | A/G |
| C05_4785547-HRM | 5 | 17.2 | GTGTAGTGCACGTCAAATAGCTG | TGGAGCATTAATTAACCACCAGAT | G/T |
| C05_10236780-HRM | 5 | 34.3 | TCCACTCTCTGTTGTGCAATACTT | AGAGATGATGGGAGAATCTCTCTG | C/A |
| C05_14937902-HRM | 5 | 38.2 | TCATCCAAACCCAACTATGTGTAG | TTATGCAGCTGAATCACATTTAGA | G/T |
| C05_11358427-HRM | 5 | 40.1 | CATGTTCTTATTCCATCCAAATCC | GCTTGTTTTAAATGCAAGAGTCAA | G/T |
| C05_9739227-HRM | 5 | 41.9 | AGACTGGTACGTGGTTCACTGTAA | AAGGAACATTCCAATCATTCTGAG | C/A |
| C05_10496220-HRM | 5 | 42.7 | GCTTAAACCTAGTGGGTGTTTCTC | AATTAAGGCATTCATTCTCCAAAC | A/G |
| C05_8684582-HRM | 5 | 46.7 | TGCAATAGACAAAGAAAACCTGAG | CTTCGATAGGTACCCACCTCAAC | A/G |
| C05_25379843-HRM | 5 | 62.3 | TTTAACTCTTGATTGGGACTAATGG | TTCTTTTACTATGGAGCAGCCAAG | C/T |
| C05_23840941-HRM | 5 | 66.5 | TTTATTTAATTTGCTACCCATCTGC | TGACCAGATCTTCGTTTATCTTCA | A/G |
| C05_23731971-HRM | 5 | 69.9 | ACAGGCGAGTATCCAATATGTTTT | TGTCTGCTCAGTATGTTGTTCAAA | A/G |
| C06_227318-HRM | 6 | 0.0 | ATTTAACCCATCCAACAAGTGATT | GTATTAAGCCTTTAGCAGCCCTTT | T/C |
| C06_505597-HRM | 6 | 7.6 | TCTTATATGATCCAAAGCCCTCAT | GCTTGCTCTGATGTATTTTCTTGA | A/G |
| C06_1893877-HRM | 6 | 11.9 | GGTGGATACATGTTTACATTGACA | CATGTCTAGGAGACTAGCAAGCAA | G/A |
| C06_2491848-HRM | 6 | 21.0 | GCAGTACCTGGTTTACTTCACCAT | CAGGCTGCAGTTTATACCAAAAGT | G/A |
| C06_3905961-HRM | 6 | 26.6 | TCTTCATCAATTACGTGCAACTCT | CAATAGTAGTGGTGGAGCAGCTTA | T/C |
| C06_4110746-HRM | 6 | 28.4 | CATCAATACAAATGAGAGCCAAAG | GGTCAGATGAAAAGCAGGTAAAGT | A/G |
| C06_4924458-HRM | 6 | 31.7 | CTTGGTTCAGCAGTCAAAAATTG | ATTGCAATATTGGTGGTTTTCTTT | C/T |
| C06_4133532-HRM | 6 | 35.5 | TACTGTATTTCTCCAGCCAAATGA | AAACATCCAAAAGCAGTACTTTCC | C/T |
| C06_5759296-HRM | 6 | 40.7 | AAGGTTAAAATACCCTTTGCCATA | TAATTACGGTTTCACTTGGAGCAG | G/A |
| C06_6012765-HRM | 6 | 42.4 | CATATATCCAATCAGCTGCCTTC | CTATTCTTTTGCCTTTGCGGTTT | C/T |
| C06_4924507-HRM | 6 | 43.0 | TCAATGAAAGAAATGGAACAAAGA | GTCAGATGGTGGTGTTAGGTTGAT | G/A |
| C06_5368959-HRM | 6 | 46.3 | ACTATTGTGCAAGCTAATGCAGTC | CAACTCTCCAATTTCCTCATCTCT | G/A |
| C06_6548604-HRM | 6 | 49.3 | TCAGGTACAACTGTCTCTTTCAGG | AAGAGAACTTCAAGTGCATTGTCA | A/C |
| C06_6885703-HRM | 6 | 52.2 | CAGAGTTGGATTGATTGTTCTTGT | ATGTATGTCTTGCCTGGGTCTACT | T/C |
| C06_8662992-HRM | 6 | 54.3 | ACGGATATTTTCATTGATGTGAGT | GAGGAAGCTCTAGCTCTGAAACTG | G/A |
| C06_8173832-HRM | 6 | 56.8 | CATTCTGGGCACTGTCATAGG | CTACCGATGTTTTACTCACTGCTG | G/T |
| C06_7866275-HRM | 6 | 58.9 | GCAAGAGTCTCACACACAGGTTTA | ATGCTGAATCCATCACCCACTAC | T/G |
| C06_7421678-HRM | 6 | 59.0 | GGTTTTCTTCATCTGCTTCTTCTG | TCCCTGAATACCCCAGTCTAATTT | C/A |
| C06_7723741-HRM | 6 | 61.8 | AATGGCGAATATGAACGTGAAC | CTGCAACCCAGGTCTGATAAAC | A/G |
| C06_14610116-HRM | 6 | 64.0 | GCATCCACTAGTCCACTTGTTTTA | CTAAAATTGATAATTGGGCTGCAA | T/C |
| C06_15260855-HRM | 6 | 66.6 | ATTAAGATCATGCAGTACGGTCAG | AGCAGACTGTTCTTATTTGTGCAG | C/A |
| C06_15788279-HRM | 6 | 69.3 | ATATCCGAACCAATCTCACTTGAC | ACTGTGGGCAGCAAATCAAT | G/A |
| C06_22090868-HRM | 6 | 73.5 | CCAAGTGTGAGAAAATTTGATGAT | CTGTCATCCCTCAGTTTCTTGG | A/C |
| C06_22554938-HRM | 6 | 74.8 | AATGCAGCAATATCATGGAACTAA | CTCATCGTACTGGCCCTCAAT | A/G |
| C07_923147-HRM | 7 | 0.0 | GTCTAATTGAAATTTTGGCTGCTC | AAAGTCCCATTATGCTTATTTTGG | C/A |
| C07_2237664-HRM | 7 | 17.7 | AGCTTGCTTAGTGATATTCACCTG | TTCTTGTTATTTAATCCCCAAAGG | G/T |
| C07_3333930-HRM | 7 | 27.6 | GTCACCCTGACATCGAACAGT | CAAAGTTGAGGATCAGTTCACACT | A/G |
| C07_5103728-HRM | 7 | 37.5 | AAAATAGATGAGAGCAAATCAGCA | AATTCAACTTGCTGCACTTATCAA | G/A |
| C07_6147311-HRM | 7 | 40.0 | TGTACAATTTCATGTTTTCGTTGTT | CTGCCTCATGTGTGCATATAACTT | T/C |
| C07_6997046-HRM | 7 | 44.3 | GAAGACCGAAGAGGTGACAATC | GACTTCCAGCTGCCTCATTC | A/G |
| C07_8012859-HRM | 7 | 46.7 | AGTTACTGCAGTTGCATGATTTGT | ATGAAAGGTCTCTTTAGCTGCAAT | G/A |
| C00_12159245-HRM | 7 | 52.6 | CACATTTGAACTCCTGATCTGTCT | AGGATGGAGAACGGTCAAACT | C/T |
| C00_11097524-HRM | 7 | 54.4 | AGTTTAAGCTTTTTGACGTGGAC | ACCCTTAACTACACGAAACATCC | C/T |
| C07_18243537-HRM | 7 | 61.3 | GATGTTTTCAGATTTGGAGTCTGG | AAATTAAGCAATACTGGACGCTTT | T/C |
| C07_20477520-HRM | 7 | 69.1 | CGACAATGAAGCTCTACTCAAAGA | CATACGCATAGTTGGCAGAATATC | A/G |
| C07_19669710-HRM | 7 | 74.8 | CACAATGTTGACGATCTAGTAGGC | GTTACAATGTGGATCAGAACTGGA | G/A |
| C07_19669455-HRM | 7 | 80.2 | GAGCCTTGATGCCTTTCATTT | TTTTACAGGTCAAAACCAAGTTGA | T/G |
| C07_22626199-HRM | 7 | 87.2 | TCTTCCTTTTCTTGAATTTGATCC | CAACAGTTGATACACTGTGTGACG | C/A |
| C07_24878394-HRM | 7 | 94.6 | ATTCCGCCAGACTCCATCAAT | ATACGAGTGTGGTAATCACCTTGA | T/C |
| C08_22356041-HRM | 8 | 0.0 | AAGGGAATCATCCAACTCATAGTC | CTCAAAAAGCAATGAAAAATGTTG | T/C |
| C08_1785228-HRM | 8 | 1.0 | TGGACTGTTTGTCTGATTTTCTGT | CAACATCTGCTATCCCAGAGAAG | C/T |
| C08_21038763-HRM | 8 | 2.8 | ATGTGGTGTGAGGTACAAGTCG | TTTCTTATGAGAATTGGAGTGCTT | C/T |
| C08_2033548-HRM | 8 | 3.9 | CTAATTTTTGCAACAAGGTTCTGA | AATTCTGCCTGGTGTATATTCAAA | T/C |
| C08_2436471-HRM | 8 | 4.4 | CCTCAACAATGATCTCCACTACAT | GTTGCTCGAGTACATTCTTTTGAA | C/T |
| C08_2063956-HRM | 8 | 4.6 | CCCTTATGCTTGTAGGAGATTCAG | CAGTTGATACCACTGGATTTGAAG | G/A |
| C08_345489-HRM | 8 | 4.8 | ATCAACAGAAGGTTGAATGACAAA | ATAAAAGCACTAACTTGGGCAGAG | T/C |
| C08_18892610-HRM | 8 | 7.4 | TTGTTTCCAATTCTTTGCTAAGTG | GACCCATGTGTTGATCCATAATC | A/G |
| C08_21108916-HRM | 8 | 8.5 | CGATGTTGCTTCTACTGCTATCTC | TGTCTGCTAACAAAGATTTCTCCTT | A/G |
| C08_4212583-HRM | 8 | 9.2 | AAAGGGTGATAGTTGGTCAGTCTC | TGAAAGAAGTTGAACTATCAGTGGA | A/G |
| C03_7003303-HRM | 8 | 12.7 | ACACTGTGATGTTTCTGGTGTTCT | CTTCACTGGGTTCATCTTCTTTG | G/A |
| C08_17766042-HRM | 8 | 14.5 | AACACGTGCACCAGGTAGTTC | GGGTCTCCTTGAACTTCTTCCTC | G/T |
| C08_4211870-HRM | 8 | 14.9 | TATGATGAGTTTGAGGAAGGTCAC | GAGCAACAGATGAATGATCAAAAC | C/A |
| C08_7860584-HRM | 8 | 20.1 | GGTTTGAAGTGTTACCATGTGTGT | ACTCACTGATTTTTGGGTGACTG | A/G |
| C08_16368863-HRM | 8 | 23.4 | ATGAATTGCTGAAACATTTTGATG | GCCAAACAATAAACACCATGTAAT | G/A |
| C08_8811114-HRM | 8 | 24.8 | GTTTCGAAGATATAAAGGGCTGTT | CAAGCAATTGAAAATTTGGTTAGA | A/G |
| C08_15737406-HRM | 8 | 26.1 | CTTTAAGGAACAAGGCTGCTTCT | GCATTGGAAGGAGAAAGAGATAGA | C/T |
| C08_15789555-HRM | 8 | 27.0 | TTTATGTGTTCTCAATTGCTGCAT | ACATTCTAACCAGTTTGCCTTGAT | G/T |
| C08_15249147-HRM | 8 | 29.9 | GTTAAGAAAGCAGCAACAATAGCA | GGTACATGTTCATTAGCCTGTCAA | C/T |
| C08_10772651-HRM | 8 | 33.1 | GGCACAACTAGGCATGTTTAATG | ATCATGCTTGACTTATGGGCTTT | T/C |
| C08_13739202-HRM | 8 | 35.5 | TTAGTTATTTCAACAGAGCCACCA | GAAGTTGAGGTAACTGGTCCTGAT | C/T |
| C08_13474335-HRM | 8 | 36.7 | CAAACCAGCTTAAACAAGAGCTTA | TATTCTTCCTGGTACCTGATCTCC | A/G |
| C08_14199614-HRM | 8 | 38.0 | CCAAGGTCAGAGAGGGTTAAAGTA | AATCCGACGAAATAATACTCCAGA | T/G |
| C08_12171467-HRM | 8 | 43.2 | CATGCTTTTCCACTTGAGACTGTA | TTTCCATGCAGATTTTACAGAAAG | C/T |
| C08_12304889-HRM | 8 | 47.2 | CATTTTCCCTTTCTCTTTTCCTAA | AAAATGAAAGGAAGCAAGTTCAAT | G/T |
| C08_11845006-HRM | 8 | 50.4 | GAATCTAGACACGGGGCTTAGTAG | GTGGAAGCTTAGTGGGTTCTTTTA | G/T |
| C08_13014955-HRM | 8 | 51.6 | CAAACTTTAAATCAAAACCCCTCA | CTCTGTTCTGTTTGTCCTGTGACT | C/T |
| C08_13105315-HRM | 8 | 57.4 | CGTAGATAGCATATACGCAGCACT | CCACCTAGTAATGTAAACCCTTGG | A/G |
| C09_56750-HRM | 9 | 0.0 | GGCCTCTCTGTTATCCTTCTCC | AGTCAAAGGAAGACCAGTTGAGAG | T/C |
| C09_241958-HRM | 9 | 1.3 | TAGAACAAGCCAGAGAAAAAGACA | ATTGTTAGGAATGCGGAAAAATAG | T/C |
| C09_911912-HRM | 9 | 8.9 | TTATTCCCTGTAGCCTTACCTGTG | CTAGTGAGACTGAAGATGGGGATG | A/G |
| C09_2355052-HRM | 9 | 19.7 | TCTGGTTTTCTAGGGATTTTGATT | ATGTACATTGCAGGAAATTGATTG | A/G |
| C09_3064878-HRM | 9 | 28.4 | TTTATGCATATCATTGAAGAAGCTG | ACAATGGTGAACATAGCAACAAAC | G/A |
| C09_3373885-HRM | 9 | 29.5 | AAAATCTAAGCAGCCATTTAGGTG | GGTGGAACAATTTGGTTTGTTTAT | T/C |
| C09_4791572-HRM | 9 | 37.4 | TTTGTTTAGGGATTTTATGCTGGT | GAGTGTGCTCTATAGCCTCATCAA | G/A |
| C09_10500656-HRM | 9 | 52.6 | ATGCAATGCACCATGTATATTACC | GAGAAGGACAGGATAAGGCTCTTT | T/C |
| C09_7464019-HRM | 9 | 54.6 | CCAGATGTATGTCTCTGGTGTCTC | AATTCCACTCTTGGCATTTGTT | A/C |
| C09_9647960-HRM | 9 | 55.6 | AATGATGAGCAGATCAATTCACTT | TATAATTGCTGCTGTAATGCTCCT | C/T |
| C09_10684763-HRM | 9 | 56.9 | GGGCAAAGGTTCTGTAATTCTTAG | CAAGTTACAGGCCATTGATAACAG | G/T |
| C09_19184208-HRM | 9 | 59.9 | TGATGTCAATGTTTTTCCACTGAT | AGCAGCTAATACATTTCCCCTACA | C/T |
| C09_16428592-HRM | 9 | 61.9 | CTCTTGCTTATGCTCTTGATGATG | AAAAGCTGCCAGAAACTCAAAAT | C/A |
| C10_322067-HRM | 10 | 0.0 | GCTCTGGCTTGTGAGGATATAACT | CAACTCTAAAGGTTTATGGGCAAA | T/C |
| C00_16798137-HRM | 10 | 7.9 | GTTGTATAAATGGTGAGGTTGTGC | ATCCATTAACTTGATCTGCAACTG | T/C |
| C00_16826464-HRM | 10 | 16.2 | TTGGAATTGTTTCAAATGTTTGTT | TCACAAAGACAAAGCCATCAGTAT | T/C |
| C10_4139927-HRM | 10 | 23.8 | GTAGCCGAGACAGGAAGTAATCAT | TGCAGCCTACTTATGTTTTCATGT | C/T |
| C00_12707874-HRM | 10 | 26.7 | GGCTAAGAATTCTAGGTCGTTGAT | GTCCACATGCTCCATCATCTC | G/A |
| C10_4648424-HRM | 10 | 28.9 | AGCTGCTTATACCAAGCTCAGG | CAGAACAGGTGATCCTTTAGTTCA | G/A |
| C10_6596875-HRM | 10 | 41.9 | GATGGAATCCAATGAAGAACAAG | CCTTCAGTAGAACCGTTATGGAGT | A/G |
| C10_10733529-HRM | 10 | 57.5 | GGCAGAGAGAACATAGTGAAGCTA | TTAGCTGCTTTATGGAGTGAACAA | A/C |
| C10_18789123-HRM | 10 | 66.3 | TATTCTTTTCACCAACAAGCTCAA | CTAGACCAACTTCTTATGGGCTTC | C/T |
| C10_19212316-HRM | 10 | 69.9 | AGCAACAGCACGCATGAC | AAAGAGGAAACGAAGGTGAAAAA | T/G |
| C10_23354864-HRM | 10 | 77.7 | AGGTAAAGAGGGACTTGTCCATTA | TTGATGCTGAATGGAGACATTAAC | A/G |
| C11_2739914-HRM | 11 | 0.0 | ATAATTTGTGGAACAGGACGAAAT | GGAGGTGTGTATCATAACTGAGCA | A/G |
| C11_1981023-HRM | 11 | 5.6 | AGAACAAACACTTAGATGCCCAAC | AGGTGGGGAAAATTGCTATTTAGT | C/T |
| C11_410719-HRM | 11 | 17.9 | AGAGGATCAAGCCTATCATAGCAG | CATGCACTAAAGGAAGGTCTTTG | C/T |
| C11_558574-HRM | 11 | 22.4 | TATAGAGAGATTTTCATGCGATGG | CTCTCTCTCTCTCTTGCCTCAAGT | T/C |
| C11_2829787-HRM | 11 | 31.1 | CACAATCTTCACAGCTACGATCTC | ATGTTTGCTCATGTGGAAGATTT | G/T |
| C11_6200534-HRM | 11 | 42.9 | GAGAAATCTTCATCACCTGCATC | CCAAAAACACAGATGGTAGTGAAA | A/G |
| C11_12546671-HRM | 11 | 56.1 | CCGACATTTTTCAACTTTAGGC | CTAGATCAAACCCAATTCCCATAG | A/G |
| C11_16840565-HRM | 11 | 67.2 | TTCCTTGTGAATTTCTCAGTAGGC | GACATTGCACCCAAAGTAGGTC | A/G |
| C11_18137435-HRM | 11 | 80.2 | AATTCAAGGAGCTGCATTCG | AAGACTTGATCTTCGGATTTGG | C/T |
| C11_19537380-HRM | 11 | 96.1 | CGTGAAGATAAAAGCAGATAACAA | ACCAAAACCTTCATCAGTACCATT | G/A |
| C12_1166573-HRM | 12 | 0.0 | CTGCAATGAAGGTTAAAACAGAAT | TAGTCAGCAGGAGATTCATAGTGG | T/C |
| C12_389448-HRM | 12 | 6.8 | AAGCATTGTCTGAGGTAACCTGTA | CATTTCATTGAACTTCTCTTGCAC | G/A |
| C12_846589-HRM | 12 | 7.1 | GGGTATTTCTGCTTTTAACAAGACC | ATCTTAAATGTCAAACTGCTGCTG | A/C |
| C12_1870128-HRM | 12 | 12.8 | TGGTGCTTTATGGTGAATACTGAT | AGAAGCCAGCATAAAAGAGAAAGA | A/G |
| C12_1632907-HRM | 12 | 13.5 | CAGAATGAGAATGATGACGATGA | CATTAGCAATTCGAGGACCTTCTA | T/C |
| C12_1764837-HRM | 12 | 14.4 | TAGAGGGAAAATTAACCAATGAGG | GCCCAGTTCCTAAGCAAGTAGTA | T/C |
| C12_3241415-HRM | 12 | 22.3 | CAACAAGGAGTGGTTTGTTAGAAG | TGTCTCAAGTGCTCTTATCACCTC | C/A |
| C12_4298131-HRM | 12 | 24.6 | AGTACATGGCTTGCCTGTCTAAG | AGGACAAGAGTCTAGGCATTTCAA | A/G |
| C12_4760456-HRM | 12 | 25.7 | AAGGGAGGACTCCACTTCACTAT | TTATCCCTTTCAACAGCTCCTAGA | C/A |
| C12_5385901-HRM | 12 | 29.5 | ATTTTAGGCTTCCTTCAGTTAGCA | TATCTGATGTCTCTTGAAGGCTCA | G/T |
| C12_6090453-HRM | 12 | 32.9 | AAGGGATGTAAAACGTCTCTTCAC | GCTTCTGCAGCTCATTTCATATT | G/A |
| C12_6212365-HRM | 12 | 33.5 | ACACAACTGTAGCTCATGCTGTTA | CAAATGCTAGAATGAAGTTGGCTA | A/C |
| C12_6420013-HRM | 12 | 35.3 | TCAAATTTCTTGGTTTATTGCATC | TGCACACTGAGAATATAACCATCC | G/T |
| C12_7104946-HRM | 12 | 39.0 | GGGAACCTGAGGAATAAGAGGATA | TATTTTGGTTTGGTTTTCAGATTG | C/T |
| C12_7351517-HRM | 12 | 41.3 | AGAACAATGGATCCAAGATTGTTT | ATAAACCGGTCTTCCTCAAGAAAT | A/G |
| C12_8261908-HRM | 12 | 44.4 | CTGCAACCTACTTTGGTAACTGCT | TCAGTTCCTTGGTTTTTGTAGTTG | G/A |
| C12_8068807-HRM | 12 | 45.7 | ATTGCTATCCTCCGGTTGG | CGGTTTCCTTTGTCGTCGT | C/A |
| C12_9268981-HRM | 12 | 49.3 | AGGGTGTAATTTGTCTCCAGCTAC | GCGGCAGTGAAGAAGGAAG | T/C |
| C12_9851926-HRM | 12 | 53.7 | AATTGCCTCTTCAAGAAATCAAAC | AGTAGAGATGATGCCAACTGTGC | T/G |
| C12_17188487-HRM | 12 | 62.6 | CTGACTTCAACACCAGGAAGATTA | TTCCCTGGATTTCAAATATAGCAG | A/C |
| C12_21717886-HRM | 12 | 72.1 | CTATGCTAGGGTTTCATCAGCAG | GAGAGCGTCACCAATCTGATCT | A/G |
| C12_23854341-HRM | 12 | 87.1 | CCTGACAATTCAGGAAACTTCATT | CTTCTCTGATCCTCTCAACATCCT | T/C |
| C13_360775-HRM | 13 | 0.0 | ATCTCCAACTTGCTTGGAAGG | TTTTTGTGGAGATTACCCTTCATT | C/T |
| C13_997503-HRM | 13 | 4.2 | GTAGGAGATGGTGGGAAGCTCT | CCAAAACAAAATAGCCTAAAATGC | A/C |
| C13_1888115-HRM | 13 | 8.8 | AATAGTGAGTTGGATGATGGCTTA | TCCCAAGGCAGAACTAAAGTTAAA | C/T |
| C13_1806932-HRM | 13 | 9.8 | GCTAGCTCCGTCCAATCCTT | AGAGCAGTGAGTAGAGACGACCTT | T/C |
| C13_21336532-HRM | 13 | 11.2 | CTTCCTGAAACCTTGAAAAAGAAG | TCTGCAGCTTCAAAACCTACTAAA | C/A |
| C13_2640974-HRM | 13 | 12.1 | CAAGCGATCTCCGTTTCTATATCT | TTTTCTTTGTCTCTCTCGATCTCA | G/T |
| C13_18522245-HRM | 13 | 13.8 | ATCCTATCGATCATGCTGACAAC | AAAGCTTTAAGTGCCTCGGTTAG | G/T |
| C13_2518720-HRM | 13 | 16.8 | ATTATAGAGGTGGTGAAGGTGGTG | ACCATTTATCTACTTCCTGCCTCA | G/A |
| C13_14811237-HRM | 13 | 17.9 | TGATGAGTTATCTACCAAGGAGCA | CACAGTAAGAGTGAAGACCTTGGA | T/G |
| C13_4082839-HRM | 13 | 20.4 | CCAAGTGTTTGACCGTTGACT | GGGTACAGTACTACCATTGGCTTC | T/C |
| C13_11410565-HRM | 13 | 23.3 | ACCCAAAAGATTGTTGCCTAGA | ATTGAACAGAGGAAAGAAGAAGGA | G/T |
| C13_5220211-HRM | 13 | 23.7 | CTGGAAACCACCTTCATAGAATTT | GCTCTTGCTGGTAGATATGGAGTC | G/A |
| C13_4884360-HRM | 13 | 27.7 | CAGTCTTTTAAGGTCGGTGACTG | CTTCTGAGGTCTTTCACCACTACA | T/C |
| C13_5865787-HRM | 13 | 28.9 | AAATTTACCATCTTACCCTTCACG | AGCTGCCACGTCACTGTACAAC | T/C |
| C13_24745554-HRM | 13 | 29.3 | CTCTTCTCACATGGAGTCTTCTCG | GTTATGAAAAAGGGTTGGAGGAG | T/C |
| C13_25253100-HRM | 13 | 29.8 | CCATCAATGCAGCAAAATAGTAAA | ATGAACCTCAAGTAACCAAGACAA | T/G |
| C13_4663382-HRM | 13 | 32.1 | TGCTGCTTTTACATGTACAGTTATG | GATCTTGAATATCAGGTTGGTAACTT | C/A |
| C13_6628718-HRM | 13 | 32.9 | CAGGCAATTCATCAACTTCAGTAT | AGGGGTAAGAAACAAGATTCCTTT | G/A |
| C13_8908781-HRM | 13 | 34.4 | GGGTGGATGTTGTTGTAACTTGTA | AGAGAATGAACGAGGTGAATGAAG | G/A |
| C13_7585688-HRM | 13 | 35.2 | ATGATGAGCTGCGGTGATATAGA | TTCTACAAGACGTGGGTAGAATCC | T/C |
| C13_3578346-HRM | 13 | 35.9 | TGCGATTTTTGTATTGAGAGTTGT | GCCACCAGCAAGTTACGG | T/C |
| C13_7807755-HRM | 13 | 36.7 | CTGTTACATGGAGTCAGAAGTTGG | TAGAAACAACAAATCGGGTAAGGT | T/G |
| C13_10484502-HRM | 13 | 38.1 | CTATCCAAAATGAACTGCTGCTAA | TCAAAGGAACTAGGTTGTATGTGC | G/T |
| C13_27377437-HRM | 13 | 38.5 | TGAATGGTATGCTGATGATAGACC | CTTGAAGCACACACTTACTCCAAT | T/C |
| C13_13237572-HRM | 13 | 39.3 | CAGTCATCTAGGTTTGGTTGAAAA | GCAGGAATAATGAAGGACATTAGG | A/G |
| C13_11097908-HRM | 13 | 40.0 | ATACACTTCATCCGTAGCTTCCTC | TCTGCATGTTATTTTGTTTCCACT | G/T |
| C18_22835365-HRM | 13 | 41.1 | GAAACAGATCATGAGTGAAAATGG | GCAAAGATAGCCCAAAAATAGAAA | A/G |
| C13_15061900-HRM | 13 | 44.1 | GTTTTGTAGGCTGCTCCTCCT | AGCCACAGGCTACAACTAAAGAAC | A/G |
| C13_19697506-HRM | 13 | 46.0 | CACTAAGAAGGTTGCATGCTAAAG | TGTTTCTCCTGCAACAAATAAATG | T/C |
| C13_25224999-HRM | 13 | 47.7 | GGTATGGGCTAGAAGATGTTGAGT | CTGAAAGTACAGCCAATGATTTGT | A/G |
| C13_23194288-HRM | 13 | 51.2 | ACTTGTGTCTTTGGAGGAGCAGT | CCAAGTTAGCACAGCCTTTTATTT | T/C |
| C13_24731492-HRM | 13 | 51.8 | TCAGAATTAGAGATTGCAGCAGAC | ATCTTCCAATGCCTATGCTTTCT | A/G |
| C13_25278544-HRM | 13 | 53.1 | TTTTCTTGTCACATTCTTCTGTGG | CTGCTGAGGTTGTTAGGCTCACT | T/G |
| C13_25652803-HRM | 13 | 54.6 | CCCCTAATGCTTCACAAAATGT | AAGTGGCATCAATGGAGGTTT | C/A |
| C13_26754940-HRM | 13 | 55.9 | GGAACTGTTCGAAGGACCTG | TCATAGACAAAAAGCACTTCAACG | A/G |
| C13_27035920-HRM | 13 | 56.7 | GCTCACACTTAGTCGTAACCATTG | ATATGCACTCATCATCGGACTGTA | C/T |
| C13_29030277-HRM | 13 | 64.4 | GGGTCCTTGAATTGATCTCATAAT | TCTCCCTTTAATCTAATGCAGCTT | G/A |
| C14_824294-HRM | 14 | 0.0 | TGCTTCTTCCGTGAATAATCCT | CTCCAGCCTGGTACATGAAAT | C/T |
| C14_1093807-HRM | 14 | 4.4 | TTTCTATTTTTGTCGCATGATGTT | TAAAATGAAAACCACCAACTTTGC | C/T |
| C14_3300294-HRM | 14 | 12.1 | TCTCTGAGTACTGAGCAAAAGCAG | ATGTGGAAGATGCAAGTAAACGTA | A/G |
| C14_5806875-HRM | 14 | 17.4 | TTGCACCTCTGTTGGAAAGTATAG | TTCCATTTATGCAGTCTATGTTCC | G/A |
| C14_7176510-HRM | 14 | 21.6 | ATTTCTTCGGTGAATAAGATCAGC | CAATTTGGAGGAGTGAAGCAG | A/G |
| C14_7207607-HRM | 14 | 22.5 | GTTCTTCACCAATTCTCGTACCTT | CCTCATCTTTCAAGCACTCTATCA | C/T |
| C14_10496252-HRM | 14 | 28.1 | GACCTTTGATGTAAATGCTGCTT | TCATGGCTTGATTTCTCTGTCTAC | C/T |
| C14_10293852-HRM | 14 | 28.6 | GATGCTTATGGTCTGTCAAGTGTT | GTATCATCCTCCATCCTCTTTTTC | C/T |
| C14_12525376-HRM | 14 | 29.6 | TCATCATGGGATGGTAACATTATT | CTGCTAAGTGATGCATTTTCTGTA | T/G |
| C14_16196404-HRM | 14 | 32.4 | AAAGGAAAGGAAAGGAAAGGAAC | CATCCACAAACCCAGACAAAT | A/G |
| C14_17812709-HRM | 14 | 34.9 | TTTGAATGTGTTCTTAACTTCTCCA | AGCAAAGAAAGCAGCAAAGTAGTC | C/T |
| C14_21067472-HRM | 14 | 41.4 | CATGCTTCATCCTCTTATCATTTTT | AATCTTTTCATTGAAGTTCGATCC | A/G |
| C14_21150740-HRM | 14 | 42.1 | ATGTCTCTGCTCCTTCAAGAACTC | AGGTGACGATATTGCACTTGG | A/G |
| C14_22893321-HRM | 14 | 49.8 | CAGATTATCAAGGCTATGGGTTG | AGGAGAGATTACAGAAGGCTGGT | G/A |
| C14_24956444-HRM | 14 | 64.0 | ACATGCATTCTCCAAATGAAACTA | AAGGGAATTGCTATGATGAAAGAG | G/A |
| C14_25421505-HRM | 14 | 67.5 | CAACAGCTAGCTTTCTTTCATCAG | CTAACCCTGAAAAACCGGAATAC | G/T |
| C14_26420306-HRM | 14 | 73.7 | ACAATCTGAAAATGAATGGGAAAT | CACCAACAATTGAGAGTGACGTAT | G/A |
| C14_29407924-HRM | 14 | 84.0 | GTGATGAACAGAGAGTCTCAAAGC | TGTGACCCACTGCATAGATCATAG | G/A |
| C14_29459475-HRM | 14 | 86.1 | ATTGATGACGTCGTCCTATTCTG | AATATTTTTCGAAGCATCAAGCTC | C/A |
| C15_1094204-HRM | 15 | 0.0 | GTTCAGACTGTTCAACACCTTCTC | GAGCTGCTAATCAAGCAATTCAT | A/C |
| C15_1094147-HRM | 15 | 2.1 | AGTGTCTTCGTCCTTCCATCTTC | AGAAGGTGTTGAACAGTCTGAACC | A/G |
| C15_325727-HRM | 15 | 3.3 | GGATAAGAGCTGTTCTGGAGAAGT | CTTTTATCTGCAACTTCATGTGCT | A/G |
| C15_401318-HRM | 15 | 4.5 | CTAGAAGCCTTCCTCTTCTCTTCA | CCACAAAAGTGTGTTTTTATGCTT | T/C |
| C15_3334519-HRM | 15 | 6.6 | AGGTTGCTGTTGGTAGTTTACTGG | GGGAGACAATTGATGAAGCTAAAC | A/G |
| C15_5414192-HRM | 15 | 7.6 | GATGATGATTGAACCCCATCTC | GACCAACAATGCTATAACAGCAAC | T/G |
| C15_11320218-HRM | 15 | 13.0 | AAAAGGATTAAGGAGGGGAAAACT | TTTGCAGCAGAGAAATCTTAATTG | T/C |
| C15_11929537-HRM | 15 | 14.9 | GTTCATCTGGTTTTACCAGTGTGT | CGCAACTTAAACCAGAAACATAAC | T/C |
| C15_13743409-HRM | 15 | 23.7 | AAATCAACCTCCACTTTTCAATTC | GTGGACTTTGGACTTTCTCTTTGA | T/C |
| C15_15000484-HRM | 15 | 28.0 | GGTCAGCACCGAGGAGTAAAC | AGCCTAGAGGGGACCTCCTT | A/G |
| C15_16459458-HRM | 15 | 35.4 | GGTTTCCAGTTCAAAGGAATGTAT | CTAGCCTCTGCTGGTTAATGCT | T/C |
| C15_17238769-HRM | 15 | 41.8 | AATCTAAAGCCTTGCACAATGAAC | GCATTTTAAAGAGCAGCAACAATA | C/A |
| C15_18031506-HRM | 15 | 47.1 | GAGTAGAACCTGAAAGCAAGCATA | AAATTCCCTTGTCTACTACTGCTGA | G/T |
| C15_17654072-HRM | 15 | 53.1 | CATCCTACAGCATTCCATTACAGA | AAGCACGTGTGATGATTCTTTTTA | T/C |
| C15_19031330-HRM | 15 | 57.6 | TTGGTTCCTAGATACCATGTCAAA | TCCTGTTAACTAACCTTGCTTCTG | T/C |
| C16_197523-HRM | 16 | 0.0 | CTTTCACCCTTCCCTCCTCT | CACTTAAATATGACCAACCACCTG | C/T |
| C16_245630-HRM | 16 | 0.7 | CACCTTCATTTTTCCATCTCTTTT | GGCTGCAAATACATCATCTCAG | A/C |
| C16_1728793-HRM | 16 | 9.9 | GAAGAAGATGCCATTGTGAAAAG | ATTTGGAACCCTAATCCACACTAC | C/T |
| C16_12729149-HRM | 16 | 15.6 | TCTTCTTTTTATTCGCATTGAACA | CTATTCCTCGAGCATCATCAATC | A/G |
| C16_4810044-HRM | 16 | 20.5 | TTGCTGCCAAAATAGATGAGATAA | GTTTCCTCAAGAAGGCTACACATT | A/G |
| C16_9654798-HRM | 16 | 22.1 | GCTTCTAGGTAGGAGGATTTCTCA | AGTGCAAACTTTGGATCACCTATT | A/G |
| C16_14572240-HRM | 16 | 28.2 | AGACATCAAAAAGGTTGAACATGA | GCTTGAAGAACCCTAATTGTGTG | A/G |
| C16_16242237-HRM | 16 | 31.6 | CTGAGTGGTGAGCTTTGGAGAG | GCTTATACCACCACTCTTTGGAAT | A/G |
| C16_19840371-HRM | 16 | 44.5 | CCTTCACCTTGTCGACTGCT | CTCAAAGGCAATTGAGGATACAAT | T/C |
| C16_22270702-HRM | 16 | 55.1 | CAATACCTATGGTGATGGATGAAA | TTTGAGATGAATTTCCAAGAGATG | C/A |
| C16_23150077-HRM | 16 | 59.4 | TATTCCAATGCAGACCTCTAACAA | GTGCAGATATGGCTTCTTTGAGAC | C/T |
| C16_23438632-HRM | 16 | 67.6 | GCTCATCATGACTGTTAGATGTCTTT | AAAACAAATAAGGTCAGCCTTGTC | T/C |
| C17_4412845-HRM | 17 | 0.0 | GCATTTGCTTATATAGATGCTGCT | GCATAGTTGCAATGTAATTTCCTG | G/A |
| C17_1853980-HRM | 17 | 4.1 | CAGAGTTTAGACAGCTCATGGAAA | AGAAGTCTCGGTTGTTCCATCTAT | A/G |
| C17_4527284-HRM | 17 | 4.6 | AGAACATGATAAATCCTCCACCAT | ATGGCCGTGTTTCACTGTCT | T/C |
| C17_4342827-HRM | 17 | 6.2 | CTCTTTTCATAAATGCTTGGGTCT | TCACCACTAGTTCATTGTGCAGTA | T/C |
| C17_3893863-HRM | 17 | 8.9 | TGCTGACTATAGACTTGCTTGAGG | CTACCTCTGGCTTCATGTGGAT | A/G |
| C17_3110117-HRM | 17 | 9.3 | ATGTCTTCCACCCACCAATATAAG | GGATGCCTGAGCACTTATGG | A/G |
| C17_2660840-HRM | 17 | 10.7 | TGCTCTAACAGGTCAGTTGGTAAA | AGCATCTGCTGCTAGCTTCATTA | T/G |
| C17_4085528-HRM | 17 | 11.5 | AGAAGCCAGAACACCCTGATATT | GTATGCATGGTTACCAATCTCCAT | A/G |
| C17_3643068-HRM | 17 | 14.3 | ACTCCATTTCATCCTTCACGTTAT | ATGACGACGGTCCAGCTC | C/T |
| C17_3088599-HRM | 17 | 21.1 | CAGCTCCTGATTGGCTTATTTATAG | TTTTTCCCAGACACCAACTAA | A/G |
| C17_7235628-HRM | 17 | 26.7 | TTTAGAGGATGAAGTCCAGTAGCA | CAATTGGAGAAATTGCTTAGTTCA | A/G |
| C17_7176322-HRM | 17 | 28.2 | ATTGAGCATGATTCTCTGTTTTGA | CAGAAAGTACCAGGACATCTGACA | G/A |
| C17_7755408-HRM | 17 | 30.6 | ACAATCACACGAGCTAAGAAATGA | CTTTGGAGATGTTTCTATCTGCTG | C/T |
| C17_10334200-HRM | 17 | 38.4 | TGGCACTTGATTAGTCTCCAATAG | ATGTTTACAGGTATTGCCCAGTCT | T/C |
| C17_9238389-HRM | 17 | 43.0 | CATTCTTGAGCCCATTTTAAGTTT | AAGGCTCAATTATTGTCCGACTAC | C/T |
| C17_11750695-HRM | 17 | 47.2 | CCAGTACTATGGGGAATCAACTGT | TGTACTCAAACAATATAGCTGCACA | C/A |
| C17_15538171-HRM | 17 | 50.1 | GATCTGTTCATGGAGATGGTGA | CTGCTTCATTCGGAATCATCTTAT | T/C |
| C17_12638800-HRM | 17 | 50.9 | GAAACATAATCTTTTTGGCAAAGG | CCATCATCATCACCATCTCATTAC | A/G |
| C17_11804262-HRM | 17 | 52.5 | GTTACCTTTCATGGAAAATGGTTC | TTTTTGCCAATTTTCTACAGTCAG | A/G |
| C17_16648079-HRM | 17 | 55.6 | CTGTTAATGCTCATAGGCATGTTG | AGTCCAACTGAGATCTATTCAGCA | T/C |
| C17_11510746-HRM | 17 | 58.3 | CGGGTCCTAAGAAGCTACCATTA | CATATTCATTTGAAAGGCGTTG | A/G |
| C17_11826795-HRM | 17 | 61.1 | CTTGCAAATGAAGAGTCTAATGCT | CTCTAAATCTCGGAGTTCTTGACC | A/G |
| C17_11019665-HRM | 17 | 65.4 | TGCTCATGTTGAACTAAAGTTGCT | TTCCCAATACAAAGTATAAACTCCA | T/C |
| C18_153959-HRM | 18 | 0.0 | ACAATTTCAAGGAGGAAGAATTTG | CATGGACATGAAGTGTTTAGGAAG | T/C |
| C18_1777529-HRM | 18 | 7.7 | TTATTTAGCTGCCACTTTTGTTTG | GTTGGTTGAGCTAGAGGATCAAAT | T/C |
| C18_907827-HRM | 18 | 11.4 | TGCTAGTGTCCTCCTAACAATCAG | CACAAGATTCTCCAACTCTTGGTA | A/C |
| C18_2575166-HRM | 18 | 14.2 | AAAGGTTCTTATGGAGCTATTTCA | TCATCAAGTGGAACTCCAAAGTTA | C/T |
| C18_352680-HRM | 18 | 15.8 | CAGAACCTTTGCATCTGCTTC | TCTCTCAAATGCGAAAGACTATCA | G/A |
| C18_200993-HRM | 18 | 16.3 | AAAAGAAGGGCATATGTAAGATGG | GGTCAATCTCTAAATGAGGTTTGG | A/C |
| C18_686672-HRM | 18 | 18.3 | CAATTTATCTCGTGGAGGCTAGTT | CAAGAAAAGCCTGATACGAAGAAT | C/T |
| C18_1670407-HRM | 18 | 21.0 | AAACCATGTGCCACACACAG | TGTAGGTTGACTGTGCAATAAAGG | G/A |
| C18_5335629-HRM | 18 | 27.1 | CTGTGAAAAATCTTCAAGGAGCAT | CAACATGACGCACCTTCTGTAATA | A/G |
| C18_3688904-HRM | 18 | 28.3 | ATTTGAAGATGCAAAAAGGAGCTA | CTTCTCCAGATGACAAGTTTCAGA | T/C |
| C18_5909613-HRM | 18 | 30.0 | AGATGAGAGGCAATGACTATGGTA | CCACTTTGATTTCTTTCATCAACA | G/A |
| C18_4162453-HRM | 18 | 31.1 | CTCTCTTTGAAGACAAAGCATCTG | GGTTAAGAACCGTGATGAAATTCT | C/A |
| C18_5131181-HRM | 18 | 33.8 | ACTAATGGCTCAATCACACCAAC | ACCCAACGAGACGCACAC | A/G |
| C18_6440306-HRM | 18 | 34.2 | GTTTTGATGGTTTGAGCATGAACT | TGATCTGTTCACCTTCTCAAGTGT | C/T |
| C18_6854075-HRM | 18 | 37.4 | GAGGATGGTGTTAACAATTGGTCT | AATTTTTCCACCTCCCATAGAGTC | G/A |
| C18_8290813-HRM | 18 | 38.7 | CTGCCTTCCAAGATCAAGAGTTAT | CTGCAGACAAAAGTAATTTCCAAT | A/G |
| C18_7384425-HRM | 18 | 40.9 | CTGCACTCGAAGTTATTACGATTT | GCAGTCGTCACAACGTATGTTAAA | C/T |
| C18_9381263-HRM | 18 | 41.7 | AGGTTCCCGAGTAATGCAGTCTT | GGAATGTTTCAGCCTATGAATTTT | G/A |
| C18_10516718-HRM | 18 | 43.5 | GATTAGAATGGTTTGAAGTTGCTG | TCTCTGAAGAAGAGGATCACCAAT | A/C |
| C18_10013638-HRM | 18 | 47.2 | CTGAAATCTTCTCCGAGCGTTT | AAATCCTTGACATGAGTTTGGAG | T/C |
| C18_13366981-HRM | 18 | 52.7 | GAAGGATCACATGACAGCTTTTT | TTTTTCAAACCCCAACAATATGTA | A/G |
| C18_14561354-HRM | 18 | 58.9 | TAACTTCAATCTCGATCTGGCTTT | CCAATCAAGACTCAGGATGTTAGA | T/C |
| C18_12969688-HRM | 18 | 66.5 | TATAATTCCCTCCAATTTGTGGTT | GAAGCAGAGTGGGCAAGTAGTAAT | A/G |
| C18_20428327-HRM | 18 | 74.0 | AAATGGCCAAGTCACTACTAAAGG | GCCCCAACACCTTAGGATTC | A/G |
| C18_17295638-HRM | 18 | 75.4 | ATCGGGATCATCAACGTCATA | TGACCATTTCATCCTTTGTGAAC | A/C |
| C18_18137477-HRM | 18 | 77.0 | TTTGATCAGAAACAAACAAGTGGT | GACAATGAGGTCTGTTGGAGTTTA | C/T |
| C18_20381800-HRM | 18 | 79.2 | CACACAGCGGTCAACGTC | GAAACTCGGACCAATCAAACC | T/C |
| C18_20847993-HRM | 18 | 79.2 | AGATGCAGTTGGTGACAAGCAT | CAAGTCTCTCTACGCCCTTGTC | C/A |
| C18_19961727-HRM | 18 | 80.8 | TACTATTATCGCCTTGCAGCCTTA | ATCGTAGAGAGCGTTTCGGTTAC | C/A |
| C18_22843850-HRM | 18 | 85.0 | ATATTTTCTGCAGGAATGCAATG | AGCTTTTAGTCCAGGAGAGATCAA | A/G |
| C18_24053356-HRM | 18 | 90.9 | CCAAATTTGTCTTACCAGAACAGG | TCTTCAGATGCACACCTAAAAATA | G/A |
| C18_23573813-HRM | 18 | 95.4 | AACCTTGTGTCTTTGTCAAGTGAA | TGGTTACAATTGTTGGGAGTTATG | C/T |
| C18_27841771-HRM | 18 | 98.2 | ATTGTGTTTTGTTTCCCTTCTCAT | AGTCTACTGAACTCTGCTGCTCAA | G/A |
| C18_27841647-HRM | 18 | 103.1 | ATTATTTGCAGCAAGAAACCTAGC | TGGTTATTGTAGGCTAATCCACCT | T/C |
| C18_32680428-HRM | 18 | 104.4 | GTGATGGTGAAGAAGATGCTAGTG | CTTTAACTGCAGTGTCACCACTCT | A/G |
| C18_29355370-HRM | 18 | 105.3 | AGTTCCTATGCATGTAATCCTTCC | TACAGTCAGACTTTTCGGGTATTG | C/T |
| C18_31821552-HRM | 18 | 107.2 | GGTTGACTGTAACCTGACATAGGC | GTATAATCAGCCACAACCAGCAT | A/G |
| C18_28308400-HRM | 18 | 109.1 | ATGGTCTCTTCGATTGCTTCAAC | GAGACAGTTACTGCCGTCGTG | G/A |
| C19_1029365-HRM | 19 | 0.0 | TTGATGCAACCAGATTATGAATG | ACTAACAGTGAAGTGAAGCAGCAG | C/T |
| C19_18490478-HRM | 19 | 7.1 | CTAAGCCATGTACCTTCCTTTCTC | CAGTAAGAGAGCTGCAAAAGCTG | A/G |
| C19_305299-HRM | 19 | 12.3 | ATTAGAAAAGCAGCTTACCCAGAA | CAATTAGGAGAGGTGAGGAATCAT | G/A |
| C19_2056568-HRM | 19 | 14.7 | CAGAAAACAATGGGAGAAGAATATC | CTCTTTGGTTTCTTTTTCTGCTTC | A/C |
| C19_1086336-HRM | 19 | 17.4 | AGACTGATGATCAATGAGATGGAA | TGTGAGTGGCTTTCAAAAAGACTA | T/C |
| C19_18490490-HRM | 19 | 21.1 | AGTGGTAAAACTTCATGGGTTGAT | CTTTAAGTTTCCACAGCTTGGTTT | G/A |
| C19_5919132-HRM | 19 | 30.4 | CAATCCTCCATGTCTTCAACCT | CATGGGTTTGGAGCAGATAGT | A/G |
| C19_18490483-HRM | 19 | 35.4 | CTGCAGTTCATATGGGAGGTTT | CTGAGATCACAGCTAAAACAAGGA | A/G |
| C19_18490491-HRM | 19 | 37.7 | TACTTAATGGCTTTGCTGAAACAG | CTGGCCTTCTCTCCTTCTCTAAG | G/A |
| C19_18490493-HRM | 19 | 47.5 | TCTGAGAACAAGAACAAAGCTTGA | TGCTAATCCTTACTTAAGCAGCAC | G/A |
| C19_10673361-HRM | 19 | 53.4 | AGATCACTTGAAAAGGCAGATACC | TCCATTTATGTGGCTTCTAGTTCC | C/A |
| C19_18490503-HRM | 19 | 56.0 | AAGTTGGTTACAGTCCCAGTTCAG | GTTTTCCTTCAACTGTGATGCTG | T/C |
| C19_18490494-HRM | 19 | 58.8 | TAGACAAAGATCACTGTTGTCAGC | AACACTATTTTCCTTGGGGAATTT | G/A |
| C19_18490506-HRM | 19 | 65.0 | GAGACCCAGCACCCTCTTC | TCAGGTACCGGAGCAAAATTAC | T/C |
| C19_14741231-HRM | 19 | 66.2 | TTTCTTACCCTCAGAATTCTCGTC | TGATTCTTTTAGGAGCCCTAATTC | G/T |
| C19_18490500-HRM | 19 | 68.0 | TGAGACGGTAAACTTCATTCTTGA | CCACCTGATGAAATAAAACCATTA | C/T |
| C19_23204606-HRM | 19 | 71.7 | TCTTTTATGTGAAAGTGGGTGATG | CATAGCAGTCCAAGAAACAACACT | G/T |
| C19_23959588-HRM | 19 | 73.4 | ATCAACCACAGTTGTGAGAACACT | ATTCTCTCCTGAACAATCTGATGC | T/G |
Summary of a genetic linkage map in an F1 population of grape cultivars ‘Tano Red’בRuby Seedless’ using HRM markers.
| Chromosome No. | Number of HRM markers | Length of linkage distance (cM) | Average marker interval(cM/marker) |
|---|---|---|---|
| 1 | 20 | 88.4 | 4.42 |
| 2 | 19 | 69.6 | 3.66 |
| 3 | 10 | 72.8 | 7.28 |
| 4 | 17 | 79.6 | 4.68 |
| 5 | 12 | 69.9 | 5.83 |
| 6 | 24 | 74.8 | 3.12 |
| 7 | 15 | 94.6 | 6.31 |
| 8 | 28 | 57.4 | 2.05 |
| 9 | 13 | 61.9 | 4.76 |
| 10 | 11 | 77.7 | 7.06 |
| 11 | 10 | 96.1 | 9.61 |
| 12 | 22 | 87.1 | 3.96 |
| 13 | 37 | 64.4 | 1.74 |
| 14 | 19 | 86.1 | 4.53 |
| 15 | 15 | 57.6 | 3.84 |
| 16 | 12 | 67.6 | 5.63 |
| 17 | 23 | 65.4 | 2.84 |
| 18 | 38 | 109.1 | 2.87 |
| 19 | 18 | 73.4 | 4.08 |
| Total | 363 | 1453.5 | 4.00 |
