This study was conducted to analyze the starch characteristics of three rice cultivars, Goami 2, Goami 3 and Goami4 with high amylose content and resistant starch. These rice varieties were developed by mutation breeding based on N-methyl-N-nitrosourea (MNU) treatment in a japonica variety, Ilpum. Resistant starch contents of three high amylose mutant rice varietiesand Ilpum were 11.87~13.69% and 0.36%, respectively. The X-ray diffraction patterns of starches of three varieties, Goami 2,Goami 3 and Goami 4 displayed B-type pattern. The proportion of amylopectin short chains of 6 to 12 in DP of the high amylosemutant rice varieties was significantly lower than that of Ilpum. Resistant starch contents had positive correlation with theamylose content, total dietary fiber content, medium length chains of 13 to 24, long chains of ≥ 25 in DP of amylopectin,pasting temperature and setback viscosity