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"culm length"

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‘Koshihikari’ is a high-quality japonica rice cultivar from Japan, exhibiting a medium-early heading type under Korean conditions, with tall plant height and susceptibility to lodging. ‘IS592BB’ is an early-maturing japonica rice cultivar in Korea, characterized by short culm, lodging tolerance, and multiple disease resistance. In this study, a recombinant inbred line population derived from a cross between ‘Koshihikari’ and ‘IS592BB’ (KSIS_RIL) was developed to investigate the genetic basis of heading date (HD) and yield-related traits through QTL analysis. QTLs associated with HD were identified on chromosomes 3, 6, and 8, and the candidate genes were assigned as Hd1 (Heading date 1), Hd16, Hd17, and Hd18. Among these, Hd1 was identified as the major QTL with the largest effect on HD variation and exhibited pleiotropic effects on multiple yield-related traits, including culm length (CL), panicle length (PL), number of spikelets per panicle (NS), ratio of ripened grain (RRG), brown/rough rice ratio (BRR), and grain yield per plant. Hd17 also affected HD, NS, and 1,000-grain weight (TGW), whereas Hd16 showed a masking effect because of interactions with other heading date genes. Combinations of alleles at Hd1, Hd16, Hd17, and Hd18 resulted in approximately 20 days of variation in HD, and specific allele combinations exhibited 4-5 days earlier heading than ‘IS592BB,’ indicating their potential for developing early-harvest cultivars. QTLs for CL were detected on chromosomes 1 and 11, with SD1 (Semi-dwarf 1) and OsCPL3 (C-terminal domain phosphatase-like 3) identified as candidate genes. SD1 was associated with variations in the CL, PL, NS, and TGW. OsCPL3 was identified within QTL associated with CL and BRR, suggesting its potential involvement in variations in plant architecture and milling-related traits. Notably, the OsCPL3allele from ‘Koshihikari’ was associated with higher BRR compared to that from ‘IS592BB,’ indicating its potential utility as a favorable allele for improving milling quality in rice breeding programs.
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국내 육성 벼 품종의 출수일수 및 간장 변이 분석
Days to Heading and Culm Length Variation of Korean Rice Varieties in Different Environments
Chang-Min Lee, Young-Ho Kwon, Hyang-Mi Park, Ji-Ung Jeung, Hyun-Su Park, Man-Kee Baek, Su-Kyung Ha, Youngjun Mo
Korean. J. Breed. Sci. 2020;52(4):389-397.   Published online December 1, 2020
DOI: https://doi.org/10.9787/KJBS.2020.52.4.389

Recent advances in high-throughput sequencing technologies have enabled large-scale cost-effective genotypic analyses, and consequently, obtaining reliable phenotypic data has now become a major bottleneck in data-driven plant breeding. In order to construct a phenotype database for commercial rice varieties released by the National Institute of Crop Science, Rural Development Administration, we initiated a systematic phenotype evaluation project, with the aim of investigating the major agronomic traits of Korean rice varieties released during the period between 1979 and 2017. Despite the narrow genetic background, we found that the days to heading (DTH) and culm length (CL) of the 297 Korean rice varieties assessed exhibited wide phenotypic variation under different environments. Under normal planting cultivation in 2018, the DTH ranged from 48 to 104 days in Suwon, 46 to 111 days in Wanju, and 39 to 97 days in Miryang, with CL values ranging from 59 to 134 cm, 55 to 122 cm, and 57 to 106 cm, respectively. During early planting cultivation in 2019, the DTH ranged from 56 to 113 days (Suwon), 58 to 109 days (Wanju), and 58 to 100 days (Miryang), with corresponding CL values ranging from 63 to 119 cm, 55 to 93 cm, and 51 to 115 cm. Despite the difference of one month in planting dates in 2018 and 2019, DTH in the different years and regions showed highly significant positive correlations (r=0.90-0.98), whereas CL showed positive but weaker correlations (r=0.45-0.82). Furthermore, we detected a weak, although significant, correlation between DTH and CL in each environment (r=-0.18-0.35). Analyses of additive main effects and multiplicative interaction (AMMI) were conducted for DTH and CL to identify rice varieties with stable phenotypes under different environments. We anticipate that the findings of this study will provide a useful rice phenotype database to facilitate genotype-phenotype association studies and data-driven rice breeding.

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