Mung bean (Vigna radiata) is a rich natural source of vitexin and isovitexin—flavonoids known for their potential anti-inflammatory properties. The aim of this study was to evaluate, through the use of RAW 264.7 macrophages, the anti-inflammatory effects of extracts from mung bean seeds containing vitexin and isovitexin. High-performance liquid chromatography (HPLC) was employed to quantify the levels of these compounds in various mung bean cultivars: “Jinhwang,” “Samhwang,” “Seonhwa,” and “Jangan,” as well as “Dahyeon,” and “Sanpo,” two of the most widely cultivated varieties in Korea. Cytotoxicity assays revealed no significant toxicity at concentrations of 25, 50, and 100 µg/mL, allowing further analysis at these levels. In nitric oxide (NO) inhibition assays, “Samhwang” (10.61 µM) and “Seonhwa” (9.7 µM) demonstrated the highest NO-suppressing activity at 50 µg/mL. Tumor necrosis factor-alpha (TNF-α) levels were significantly reduced by “Seonhwa” (83.6 pg/mL) and “Jangan” (72.3 pg/mL), with “Jangan” showing the strongest inhibitory effect. Interleukin-6 (IL-6) analysis revealed notable suppression in “Samhwang,” “Seonhwa,” and “Jangan” at 50 µg/mL, with “Samhwang” exhibiting the most potent effect (78.6 pg/mL). These findings suggest that the “Samhwang” cultivar, in particular, possesses significant anti-inflammatory potential and may serve as a valuable candidate for the development of natural anti-inflammatory agents.