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Volume 53(4); December 2021

Articles

유전자교정 기반 신육종기술의 글로벌 특허동향
Global Patent Trends in New Breeding Technology for Crop Improvement
Serry Koh, Youri Choi, Joo Young Lee, Jiyoung Jang, Kyuwhan Choi
Korean. J. Breed. Sci. 2021;53(4):337-349.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.337

New Breeding Technology (NBT) refers to gene editing technologies that are used to develop crop plants with beneficial traits, from biotic/abiotic resistance to nutritional enhancement, including zinc finger nucleases (ZFN), transcription activator-like effector nucleases, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9, meganucleases, and oligo directed mutagenesis. A total of 1,119 valid NBT patents were analyzed in this study to examine global trends in the patent and market expansion strategies for major patent applicants. Based on the claims specified, valid patents in each patent office were analyzed through the applicant’s country of origin, field of technology, and plant/crop species. Patents claiming applications of CRISPR-based technology to major crop plants, including rice, corn, wheat, tomato, and canola, have rapidly increased in the China National Intellectual Property Administration (CNIPA) since 2013. The patent family size (PFS) can be used as an indicator of intellectual property (IP)-based market expansion strategies and target markets of interests of patent applicants. Many university- and research-oriented institution Chinese applicants showed low PFS (2.1) because they filed patents mostly in CNIPA. In contrast, high PFS of US and German (DE) applicants such as Corteva Agriscience (US), KWS SAAT AG (DE), Cellectics (FR), and Syngenta Participations AG (CH) represented their active strategies for global gene-edited crop market expansion. Corteva Agriscience (US, 238 patents) was the global leader in patents using NBT, ranging from ZFN to CRISPR-based technologies applied to most major crops, including corn, soybean, and wheat.

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복숭아 NGS 분석에 의한 다형성 SSR 마커 개발과 활용
Anticipated Polymorphic SSRs and Their Application Based on Next Generation Sequencing of Prunus Persica
Jung Sun Kim, Yoon Suk Ku, Sin-Gi Park, Se Hee Kim, Hyun Woo Park, So Youn Won
Korean. J. Breed. Sci. 2021;53(4):350-360.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.350

Prunus persica “Mihong” cultivar is a domesticated white peach that was generated from the crossing between “Yumyeong” and “Chiyomaru” cultivars in the Republic of Korea in 1995. We launched “Mihong” genome sequencing in 2018 and “Mihong” reached to 200 scaffold and 241 Mb sequences using long-read sequencing and Hi-C technology. F1 populations of ”Kawanakajima Hakuto,” “Mihong,” “Changhowon Hwangdo,” and “Yumi” were developed in NIHHS. These four cultivars were sequenced and assembled using the SOAPdenovo version 2.04. First, we surveyed the SSRs in “Mihong” assembly sequences and extracted the ±300 bp flanking sequences containing SSRs. Second, the assembly sequences of three cultivars were aligned and mapped against “Mihong” ±300 bp flanking sequences using BLASTn (version 2.2.29+). We anticipated the differential length in SSRs among the four cultivars. We sorted the primers with a standard deviation over 4.5 (STEV > 4.5) among the four cultivars. In addition, we surveyed the primers having difference in over 10 bp with “Kawanakajima Hakuto” and “Mihong” for polymorphic markers in the mapping population. All primer pairs were designed to generate amplicons of 150-200 bp in coating SSR regions using primer3 (version 3-2.2.3). We selected 260 SSR markers with a physical distance of average per 1 Mb. These SSR markers accounted for 74% polymorphism in the four genotypes. Finally, a F1 population of “Kawanakajima Hakuto” and “Mihong” covered 884.5 cM with 465 SNPs and 86 SSRs and this genetic map matched correctly to the HI-C pseudomolecule of P. persica.

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유전자 좌위 분자마커를 이용한 아밀로스 함량별 벼 품종 분류
Classification of Korean Rice Cultivars through Amylose Content Using Molecular Markers Targeting the Waxy Locus
So-Myeong Lee, Young-Ho Kwon, Ju-Won Kang, Ji-Yoon Lee, Sumin Jo, Dongjin Shin, Jin-Kyung Cha, Dong-Soo Park, Jun-Hyeon Cho, Jong-Hee Lee
Korean. J. Breed. Sci. 2021;53(4):361-372.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.361

A total of 285 Korean rice cultivars were used for amylose content and genetic analyses using KASP markers related to the amylose content. We used three polymorphic KASP markers targeting the Waxy (Wx) gene and compared the genotyping efficiency with previously reported molecular markers. The rice cultivars were raised in Miryang, Korea, for one period. Genetic analysis of the rice cultivars revealed that the three KASP markers on the Wx gene were functional for genomic selection of SNPs related to amylose content variation. Therefore, the three KASP markers targeting different Wx alleles can be used for genomic selection. In addition, KASP markers were more efficient than previously reported molecular markers that target the same alleles. These findings can be beneficial for the breeding and genomic selection of high-quality rice in Korea.

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Interspecific hybridization is a valuable approach to generate genetic variation and to introgress desirable genes. The objective of the current study was to estimate the heritability and correlation coefficient of several fruit traits for three years (from 2016 to 2018) using a set of 178 interspecific F1 populations developed from a cross between ‘Manpungbae’ (Pyrus pyrifolia) and ‘Oharabeni’ (P. pyrifolia×P. communis). For ‘Manpungbae’, the average values of fruit weight, length, diameter, fruit shape index, and soluble sugar content for the three years were 967.8 g, 109.0 mm, 125.7 mm, 0.87, and 13.4 °Brix, respectively. The average values for ‘Oharabeni’ were 268.2 g, 75.2 mm, 81.4 mm, 0.93, and 9.9 °Brix, respectively. The average values and heritability for three years in F1 progenies were 222.0 g and 85.55%, 67.3 mm and 84.14%, 75.4 mm and 81.18%, 0.89 and 79.33%, and 10.7 °Brix and 77.75%, respectively. Among the five fruit traits we surveyed, the Pearson’s correlation coefficients between three traits (weight, length, and diameter) were highly significantly positive (over 0.8) for the three years.

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다양한 비생물적 스트레스에 의해 유도되는 프로모터의 특성분석
Characterization of the RsMYB1 Promoter Induced by Various Abiotic Stresses
Da-Hye Kim, Ju-Hee Yang, JuHee Rhee, Jong-Yeol Lee, Sun-Hyung Lim
Korean. J. Breed. Sci. 2021;53(4):380-391.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.380

Plants grown under stress conditions generate excessive reactive oxygen species resulting in cell death. Therefore, plants activate the protection mechanism via antioxidant accumulation. Anthocyanins are flavonoid-derived secondary metabolites with high antioxidant properties. In this study, we analyzed and characterized the promoter region of RsMYB1, a positive regulator of anthocyanin biosynthesis. The RsMYB1 promoter was designed with four different fragment lengths (MP1, -1034; MP2, -830; MP3, -633; and MP4, -430 bp), and then each RsMYB1 promoter region was fused into a GUS gene for Arabidopsis transformation. The expression patterns of the RsMYB1 promoter constructs were analyzed at different developmental stages and under various abiotic stresses. The GUS expression pattern steadily increased with plant growth, and coincided with enzyme activity and a histochemical GUS assay. In response to drought, salt, sucrose, and low temperature, the GUS transcript level was highly expressed in MP4 in parallel with GUS enzyme activity. These assays indicated that the proximal region (-430 to -1 bp) of RsMYB1 was the core sequence that was induced by salt and low temperature. The expression level of RsMYB1 in the leaves of radish was highly activated and was consistent with the anthocyanin content under salt and low temperature conditions. These results suggest that induction of the RsMYB1 gene can activate the biosynthesis of anthocyanins, which are expected to help plants adapt to stress conditions due to their antioxidant activity.

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저항성 유전자 도입과 벼 후대계통의 열악형질간의 관계분석
Effect of Resistance Genes on the Occurrence of Rice Undesirable Characters in a Wide Cross
Chang-Min Lee, Hyun-Su Park, Man-Kee Baek, Jung-Pil Suh, O-Young Jeong, Song-Joong Yun, Suk-Man Kim
Korean. J. Breed. Sci. 2021;53(4):392-403.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.392

Interspecific and intersubspecific crosses have been an important strategy for rice breeding programs to obtain useful characters by expanding genetic diversity. However, the wide cross routinely causes undesirable characters in rice, such as spikelet sterility and poor quality. In this study, we developed near-isogenic lines of R-gene (Xa4+xa5+Xa21+Pi40+Bph18) introgression population to investigate the genetic relationship between the occurrence of poor characters and R-genes induced by MAS. The ANOVA analysis revealed that Pi40, Bph18, and Xa4 were associated with spikelet fertility (SF), ratio of ripened grain (RRG), third internode length (TIL), panicle extraction (PE), and protein content (Pro), whereas others showed no association with the 14 traits tested. In the three-way ANOVA, the impact of two R-genes (Pi40 and Bph18) on the poor characters was independent, with no interaction with others. Quantitative trait locus (QTL) analysis identified QTLs for PE and TIL traits near Pi40, and in particular, QTLs for five traits (SF, RRG, TIL, PE, and Pro) were negatively affected around Bph18. The occurrence of a poor character in R-gene pyramiding lines could be associated with linkage drag caused by the Bph18 gene. We speculate that the development of DNA markers that effectively eliminate poor characters will not only improve breeding efficiency using MAS, but will also help improve traits of japonica rice through wide crossing.

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멜론 덩굴쪼김병균( f. sp. ) race 2에 대한 멜론 유전자원 저항성 평가
Evaluation of Melon Germplasms Resistance to Fusarium oxysporum f. sp. melonis race 2
Aejin Hwang, Hyungjun Noh, Onsook Hur, Nayoung Ro, Jung-Yoon Yi, Jae-eun Lee, Bichsaem Kim, Ji Hyeon Kim
Korean. J. Breed. Sci. 2021;53(4):404-410.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.404

Fusarium wilt is a serious disease that causes damping-off, serious wilt symptoms, or death in melon. The aim of this study was to evaluate the resistance of melon germplasm to Fusarium wilt and select promising disease-resistant accessions for further applications. Resistance to Fusarium wilt was examined in 216 melon germplasms by the root-dipping inoculation method with Fusarium oxysporum f. sp. melonis fungal isolate KACC43206 (race 2). Inoculated seedlings were incubated for four weeks and assessed for disease symptoms using indices ranging from index 1 (no symptoms) to 5 (plant wither). A total of 34 accessions showed a disease index < 2.0. Disease indices of 10 accessions were 1.0 four weeks after inoculation. The resistant accessions of melon are expected to help in breeding commercial varieties with Fusarium wilt resistance.

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한국 재래종 콩 유전자원의 이소플라본 함량과 수량관련형질에 대한 연차간 비교
Yearly Variation of Isoflavone Composition and Yield-Related Traits of 35 Korean Soybean Germplasm
Hyemyeong Yoon, Jungyoon Yi, Kebede taye Desta, Myong-Jae Shin, Yoonjung Lee, Sukyeung Lee, Xiaohan Wang, Yu-Mi Choi, Sungwoo Lee
Korean. J. Breed. Sci. 2021;53(4):411-423.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.411

In this study, composition and agronomic traits, including yield-related traits, were investigated and compared among 35 Korean landrace germplasm for two years (2019 and 2020). The contents of 12 isoflavones were measured using high-performance liquid chromatography, and agronomic trait data were collected according to the Rural Development Administration criteria. Comparing isoflavone composition between the two years, all isoflavones, except glycitein, were generally higher in 2019. The values of yield-related and major agronomic traits decreased in most accessions in 2020. ANOVA showed highly significant differences (p<0.001) in genotypes, years, and genotype-by-year interaction for all isoflavones, the number of pods per plant, and 100-seed weight. The number of seeds per pod, days to flowering, and days to maturity also showed significant differences among genotypes, as well as between the two years. Correlation analysis revealed that daidzin and genistin were positively correlated with their malonylated form. Malonyl daidzin was also positively correlated with malonyl genistin levels. The accessions with the least variation in the total isoflavone content were IT 153844 (907.5 µg/g, 898.6 µg/g) and IT 252252 (663.2 µg/g, 636.4 µg/g). Some of the evaluated landraces will be a promising genetic source for developing soybean varieties with high levels of environmental stability in isoflavone content and agronomic traits.

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한국 재래종 벼 유묘기 뿌리 발달 전장유전체연관분석
Genome-Wide Association Study of Root Development at the Seedling Stage in Korean Landrace Rice
Jeonghwan Seo, Hongjia Zhang, Ah-Rim Lee, Wondo Lee, Yoo-Hyun Cho, Soon-Wook Kwon, Joohyun Lee
Korean. J. Breed. Sci. 2021;53(4):424-431.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.424

Root development at the seedling stage is an important trait for the early growth of rice. In this study, the root length and weight of 211 Korean landrace rice at the seedling stage were evaluated, and the average root length and weight were 6.94 cm and 48.18 mg, respectively. Principal component analysis (PCA) of 211 accessions based on 70,849 SNPs presented three subgroups. Two quantitative trait loci (QTLs) associated with root length were detected on chromosomes 3 and 6 using genome-wide association study (GWAS). Four haplotypes were identified using associated SNPs for the two root length QTLs. The average root length of Hap2 and Hap 3 was 7.32 cm and 6.56 cm, respectively, and were significantly different. Gene expression analysis in the candidate regions of QTLs for root length at the seedling stage showed that several genes were expressed in the root. QTLs associated with root length at the seedling stage identified in this study may be applied to improve the root length trait and detect candidate genes.

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Fluidigm 시스템을 이용한 고추 유전자원의 주요병 유전형 평가
Evaluation of Genotypes for Major Diseases of Pepper Genetic Resources Using the Fluidigm System
Bora Geum, Na Young Ro, On sook Hur, Xiaohan Wang, Yu-Mi Choi, Hocheol Ko, Sukyeung Lee
Korean. J. Breed. Sci. 2021;53(4):432-449.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.432

In this study, genotype analysis was conducted using the Fluidigm system with 13 SNP markers related to anthracnose, powdery mildew, phytophthora root rot, cucumber mosaic virus (CMV), potyvirus, and tobacco mosaic virus (TMV) resistance. A total of 25,350 data points were obtained, 8.2% of which indicated resistance genotypes. Resources having perfect resistance genotype to each disease-related molecular marker set were 58 accessions for anthracnose, 57 for powdery mildew, 246 for phytophthora root rot, and 765 for CMV. There was no resource having a perfect resistance genotype of all three potyvirus-related molecular SNP markers including pvr1, pvr2 (123457), and pvr2 (689). Thirty-seven accessions for pvr1, 349 accessions for pvr2 (123457), and 23 accessions for pvr2 (689) were identified as resources with resistance genotype. No resource with the TMV-resistance genotype was found in this study. The resources with complex resistance genotypes of anthracnose and powdery mildew were 55 accessions, and they were mainly Capsicum baccatum. Among these, seven accessions had a three-disease combined resistance genotype with phytophthora root rot. Thirty-six accessions, 16 accessions, and nine accessions each had resistance genotypes of the three potyvirus-related markers. These resources also had CMV and phytophthora root rot resistance genotypes, and they were mainly Capsicum chinense. The statistical analysis considering the evaluated resistance genotypes, revealed that the anthracnose resistance genotype showed a tendency to be linked with powdery mildew resistance genotype. The potyvirus-related marker pvr1 was positively correlated with Phytophthora root rot markers.

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작물 표현체 플랫폼 기반 벼 이미지 분석 조건 확립
Determination of the Conditions for Image Analysis of Rice Based on a Crop Phenomic Platform
Chaewon Lee, Inchan Choi, Hongseok Lee, Nyunhee Kim, Eunsook An, Song Lim Kim, Jeongho Baek, Hyeonso Ji, In-Sun Yoon, Kyung-Hwan Kim
Korean. J. Breed. Sci. 2021;53(4):450-457.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.450

Fast and accurate selection is essential for breeding to cope with rapid climate changes and a steeply increasing population. Consequently, technologies for high-throughput phenotyping (HTP) are emerging. These technologies, unlike conventional phenotyping methods, enable us to evaluate agronomic traits in a fast and massive manner. Thus, the HTP facility was built to acquire and analyze crop images using RGB sensors at the National Institute of Agricultural Sciences, Republic of Korea. By testing various conditions to acquire images, we determined the conditions for phenotyping using the RGB sensor as follows: exposure 30,000 ms, gamma 75, and gain 100 using LED lights in a blue background. Based on this condition, images from 96 individual plants of rice Dongjin cultivar were obtained every week to measure plant height and shoot area, which are directly associated with yield. The results obtained from the image analysis were compared with the manually collected results. The r2 value between the projected plant height obtained from image analysis and the plant height obtained from manual measurement was 0.989. Furthermore, the r2 value between the projected shoot area obtained from image analysis and the shoot area obtained from manual measurement was 0.981. These results show that image analysis is highly reliable and can be used for crop phenotyping. Therefore, we expect that the new method we developed will be used for breeding in the near future.

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New Cultivar Developed
저온에서 녹기연장의 한국잔디 ‘한라그린 7’ 품종 개발
Development of ‘Halla Green 7’: A Zoysiagrass Cultivar with Greening Period Extending Phenotype at Lower Temperatures
Dae-Hwa Yang, Hyeon-Jin Sun, Ok-Cheol Jeong, Hong-Gyu Kang, In-Ja Song, Il-Doo Jin, Si-Yong Kang, Hyo-Yeon Lee
Korean. J. Breed. Sci. 2021;53(4):458-466.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.458

The ‘Halla Green 7’ cultivar is a hybrid of Zoysia matrella and Zoysia japonica. The inflorescences of Z. matrella lines were collected from Jeju Island near the border between Z. matrella and Z. japonica wild plants, respectively. After their harvest, the seeds were sown and various interspecific lines were obtained. In 2021, a line with greening period extending phenotype during the low temperatures of winter was registered under the cultivar name of ‘Halla Green 7’ (Registration No. 245). The main morphological characteristics of the ‘Halla Green 7’ variety are as follows: genetically short length of plant (12.2±0.58 cm), very wide angle of leaf (68.6°), short length of leaf blade (8.38±0.37 cm), medium-leaf (3.29±0.09 mm), and very low leaf sheath (2.18±0.19 cm). The short length of leaf sheath makes it easy to manage the turf grass with low height. A very wide leaf angle has a high photosynthetic efficiency. The ‘Halla Green 7’ cultivar is morphologically classified as Z. matrella×Z. japonica hybrid line by medium-width of leaf blade and the presence of trichomes on the leaf surface. The colors of the leaf blade, leaf sheath, and stolon are green, light green, and light purple, respectively. During the low temperature period of winter, chlorophyll content of the leaves of ‘Halla Green 7’ shows a 10.1-fold from that of the control Z. japonia ‘Yaji’ and about 4.2-fold from that of the control Z. matrella Zm-6. This greening period extending phenotype is expected to contribute to the industrialization of zoysiagrass (Registration No. 245).

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한국잔디 ‘한라그린 10’ 품종의 개발
Development of Cultivar ‘Halla Green 10’ in Zoysiagrass
Dae-Hwa Yang, Ok-Cheol Jeong, Hyeon-Jin Sun, Hong-Gyu Kang, Il-Doo Jin, Hyo-Yeon Lee
Korean. J. Breed. Sci. 2021;53(4):467-474.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.467

This superior zoysiagrass line was selected from the zoysiagrass genetic resources collected in the Republic of Korea. Inflorescences of Zoysia matrella lines were collected near the border between wild Zoysia japonica and wild Z. matrella on Jeju Island. Various Z. matrella×Z. japonica lines were obtained using the seeds from the collected inflorescences. In 2021, one of the lines that extended the greening period during the low temperatures of winter was registered under the name of ‘Halla Green 10’. The main morphological characteristics of this cultivar, which were analyzed using the test guidelines of the Korea Forest Service, are as follows: short length of plant (12.01±0.57 cm), short length of leaf blade (8.83±0.68 cm), and short length of leaf sheath (1.71±0.20 cm). The short length of leaf sheath makes it easy to manage the turf grass with low height. Due to the presence of trichomes on the leaf surface and medium-leaf type (leaf blade width: 3.0±0.16 mm), ‘Halla Green 10’ is morphologically classified as Z. matrella×Z. japonica. As the leaf angle is very wide at 77.7°, it has a high photosynthetic efficiency. The colors of the leaf sheath, leaf blade, and stolon are light green, green, and light purple, respectively. In particular, during the low temperature period of winter, the chlorophyll content of the leaves of ‘Halla Green 10’ shows about 7.5-fold from that of the control Z. japonia ‘Yaji’ and about 3.2-fold increase from that of the control Zm-6. Extending the greening period phenotype is expected to be commercially useful (Registration No. 246).

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‘Sunmyoung’ is a high-yielding variety that is resistant to damage from lodging and high temperatures. Out of the 30 lines collected from a farmhouse in Yeoju in 2001, individual plants of ‘Sunmyoung’ were selected from a testing plot with artificial high-temperature stress and were further bred using the pure line separation breeding method. Preliminary and advanced yield trials were conducted in Suwon and Yeoju from 2005-2013, and regional adaptability trials were conducted in Yeoju and Anseong from 2014-2016. The rates of high-temperature stress for two- and three-year old ‘Sunmyoung’ were lower than those of the ‘Sunil’ variety by 46.2% and 34.1%, respectively. The length of the stem and small leaflets of Sunmyoung were 6.2 cm and 5.1 cm shorter than those of Sunil, and the width of the leaflets was shorter than that of Sunil by 3.9 cm-indicating a higher resistance of ‘Sunmyoung’ against lodging damage. In the regional adaptability test, the yield of Sunmyoung was 523 kg/10a, which was 5% higher than that of ‘Sunil’ (498 kg/10a). The content of ginsenoside in ‘Sunmyoung’ was 19.2 mg/g, which was lower than that of ‘Sunil’ (22.38 mg/g). Since ‘Sunmyoung’ is more resistant to high temperature stress (than ‘Sunil’), lodging stress, Rhizoctonia solani, and Alternaria panax leaf spot, and has a decent production stability with higher yields, it is expected to contribute towards the quality improvement of raw materials in ginseng production (Registration No. 7495).

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내건 내서성 감자 ‘아리랑 2호’
‘Arirang-2’: A Potato Variety Tolerant to Drought and Heat Stress
Young-Eun Park, Ji-Hong Cho, Dong-Chil Chang, Jang-Kyu Choi, Hyun-Jin Park, Jin-Hee Seo, Gyu-Bin Lee, Ju-Sung Im
Korean. J. Breed. Sci. 2021;53(4):482-488.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.482

In 2018, a new potato variety, ‘Arirang-2’, was released by the Highland Agriculture Research Institute in Pyeongchang, Korea. To create new varieties with better tuber formation at high temperature under long day, a cross was performed between high-temperature tolerant ‘Eigenheimer’ and drought tolerant ‘Daegwan 1-116’ in 2011. The shoot (57.9 cm) of ‘Arirang-2’ was taller than ‘Superior’ and ‘Sante’, and its tuber skin and flesh were red and light-yellow colored, respectively. Average number of tubers per plant of ‘Arirang-2’ was 9.1 while ‘Superior’ and ‘Sante’ had 5.4 and 14.1 tubers, respectively. Average weight of tuber of ‘Arirang-2’ was 98.1 g, which was slightly heavier than that of ‘Superior’ (90.0 g), and much heavier than that of ‘Sante’ (38.9 g). Total weight of tuber per plant of ‘Arirang-2’ was 892 g, which was remarkably higher than that of ‘Superior’ (486 g) and ‘Sante’ (549 g). Total yield (TY) of ‘Arirang-2’ measured in Gangwon-Do, Korea was around 5,022 kg/10a while that of ‘Superior’ was 3,036 kg/10a. In Kazahstan, total yield of ‘Arirang-2’ was 4,657 kg/10a and that of ‘Sante’ was 4,155 kg/10a. Marketable tuber yield (MTY) of ‘Arirang-2’ was 3,119 kg/10a in Gangwon-Do, Korea while that of ‘Superior’ was only 2,147 kg/10a. In Kazahstan, MTY of ‘Arirang-2’ was 3,162 kg/10a, which was 60% higher than that of ‘Sante’ (1,974 kg/10a). ‘Arirang-2’ is resistant to early blight (Alternaria solani) and late blight (Phytophthora infestans) compared to ‘Superior’ and ‘Sante’, and it shows tolerance to drought or high temperature. ‘Arirang-2’ was approved as a new potato cultivar by The Korea Seed and Varieties Service on March 3, 2021 (Registration No. 8444).

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담적색 종피색의 내도복 신품종 팥 ‘홍경’ 육성
An Adzuki Bean (Vigna Angularis) Cultivar ‘Honggyeong’ with Bright Red Seed-Cort Color and Resistance to Lodging Stress
SeokBo Song, MyeongEn Choe, JiHo Chu, JiYong Kim, ByongWon Lee, SangIk Han
Korean. J. Breed. Sci. 2021;53(4):489-494.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.489

In 2008, a new adzuki bean (Vigna angularis) cultivar, ‘Honggyeong’, was developed using artificial cross between ‘Milyang 7 (SA9709-2B-8-2-4-4-1)’ and ‘SA0001’ at the National Institute of Crop Science, RDA, Korea. It was selected for further yield trials-named ‘Miryang 36’. This cultivar was prominent and showed desirable agronomic traits, such as lodging tolerance, high yield, and high antioxidant activity during the regional adaptation yield trials (RYT) from 2016-2018. ‘Honggyeong’ was finally released as a new cultivar in 2018 and its distinct phenotypic traits included a semi-determinate growth habit, yellow flowers, green embryonic axis, bright red seed-cort color, white hilum, and large seeds (18.4 g per 100 seeds). The average yield of ‘Honggyeong’ was 2.06 MT/ha in the regional yield trials (RYT), which was slightly higher than that of the check cultivar (1.89 MT/ha), ‘Chungju’ (Registration No. 7934).

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조생종 고품질 완전단감 ‘원추’ 육성
Breeding of Early Maturing and High-Quality Sweet Persimmon: ‘Wonchu’
Kyeong-Bok Ma, Sang-Jin Yang, Ye-Seul Jo, Kwang-Sik Cho, Sam-Seok Kang
Korean. J. Breed. Sci. 2021;53(4):495-499.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.495

In 2005, a new persimmon (Diospyros kaki L. Thunb.) cultivar ‘Wonchu’ was derived by crossing ‘Shinsyu’ and ‘Taishu’, and it was finally selected in 2015. In the Yeoung-am province, the average maturing date of the fruit of this cultivar was October 3rd. The quantitative characteristics of the fruit of ‘Wonchu’ include a larger size (324 g on average) and presence of high soluble-solids (approximately 15.1 °Brix). The flesh of the fruit is juicy, has a crispy texture, and tastes good. The shape of the fruit is round oblate (when looked from the side), and the color of its skin is orange. Physiological disorders, such as fruit cracking or fine skin cracking, rarely occur (Registration No. 8579).

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다분지 고올레산 다수성 땅콩 신품종 ‘해올’
‘Hae-Ol’: A Virginia-Type High-Oleate Peanut Variety
Eunyoung Oh, Suk-Bok Pae, Sungup Kim, Jung-In Kim, Min Young Kim, Jeongeun Lee, Sang Woo Kim, Kwang-Soo Cho, Myong-Hee Lee
Korean. J. Breed. Sci. 2021;53(4):500-507.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.500

‘Hae-Ol’ (Arachis hypogaea ssp. Hypogaea) -a high-oleate peanut variety-was developed at the Department of Southern Area Crop Science, National Institute of Crop Science in 2018. This variety was selected from the pedigree line crossed between short stem Virginia-type ‘HP825’ and high-oleate elite line ‘YG316’. The crude fat content of ‘Hae-Ol’ was 50.8%, and its oleic acid composition was 83.0% showing high-oleate property compared to that of ‘Daekwang’ variety (57.3%). The ratio of oleic acid to linoleic acid, which represents the oxidative stability, was 12 times higher in ‘Hae-Ol’ than in ‘Daekwang’. The increments in acid value and peroxide value were relatively slower in ‘Hae-Ol’, under the condition of accelerated oxidation. Over the three years of regional adaptation test (2016-2018) conducted at four regions, ‘Hae-Ol’ showed significantly higher grain yield (4.88 MT⋅ha-1), which was 16% more improved than that of ‘Daekwang’ (4.20 MT⋅ha-1). The seed of ‘Hae-Ol’ is brown in color and long-ellipse in shape and its 100-seed weight was 96 g. On field, ‘Hae-Ol’ demonstrated more resistance to late leaf spot (Cercosporidium personatum) and web blotch (Phoma arachidicola). It also had a shorter branch and main stem, which was related to the higher tolerance to lodging stress. The release of ‘Hae-Ol’ could contribute to the production of peanuts with enhanced quality and higher yield, and it is expected to be notably beneficial to both the producers and manufacturers (Registration No. 8407).

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수출용 대과성 딸기 품종 ‘알타킹’
‘Altaking’: An Exportable Strawberry Variety with Large-Sized Fruit
Mingu Kang, JongDo Cheung, DoYeun Won, JaeSoon Hwang, HanWoo Do, HongJib Choi
Korean. J. Breed. Sci. 2021;53(4):508-514.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.508

In 2013, a strawberry variety ‘Altaking’ was derived as an artificial cross between ‘12-S-4’ (with high firmness) and ‘Ssanta’ (with high sugar content). Seedling and line selections were conducted from 2013-2015. Preliminary and advanced yield trials of ‘13-S-6-3,’ which was the final selected line, were conducted from 2015-2017. The general characteristics of ‘Altaking’ include upright growth habit, elliptical leaves, and strong vigorous growth. The fruit is long, conical, and light-red in color. The average number of flowers per flower cluster was 12.1, which could reduce the labor required for fruit thinning. This variety is suitable in forcing culture because of its early flower-bud differentiation (September 1), early first harvest (mid of November), and continuous formation of the next flower cluster. ‘Altaking’ has adequate characteristics for exportation because the sugar content rises in the early stages of color development. Although ‘Altaking’ and ‘Seolhyang’ have similar sugar content, the former is less sour, thus making it taste sweeter. ‘Altaking’ has a unique flavor and it smells like peach. The fruit weighs approximately 35.1 g, and it should be harvested at 80% coloration to intensify its deep flavor. In 2019, ‘Altaking’ was registered with the Korea Seed and Variety Service (KSVS) for plant variety protection (Registration No. 7854).

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중만생 복합내병 다수성 발아현미용 거대배아미 ‘큰품’
‘Keunpum’: A Mid-Late Maturing, High Yielding, Giant Embryo Rice Cultivar with Resistance to Multiple Diseases and Used as Germinated Brown Rice
Eok-Keun Ahn, Ung-Jo Hyun, Kuk-Hyun Jung, Yong-Jae Won, Ha-Cheol Hong, Hyang-Mi Park, Jae-Ki Chang, Jeong-Heui Lee, Jeom-Ho Lee, Nak-Sig Sung, Jung-Pil Suh, Sea-Kwan Oh, Mi-Ra Yoon
Korean. J. Breed. Sci. 2021;53(4):515-525.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.515

‘Keunpum’ is a mid-late maturing, high yielding, giant embryo rice (Oryza sativa L.) cultivar which is adapted to central plains and was developed to be used as (germinated) brown rice. It is resistant to multiple diseases with a good grain quality. This cultivar was derived by crossing a giant embryo rice variety ‘Keunnun’ and a mid-late maturing, multi-disease resistant rice cultivar ‘Samkwang’-it has good palatability of cooked rice. The growth period of this cultivar is approximately 117 d from seeding to heading, with a culm length of 88 ㎝, panicle length of 20 ㎝, 13 panicles per hill, 136 spikelets per panicle, and 1,000-grain weight of 19.1 g brown rice. This variety has low tolerance to cold stress and premature germination similar to ‘Keunnun’ but exhibits high resistance to lodging. In addition, ‘Keunpum’ is resistant to bacterial blight (race K1, K2, K3), rice stripe virus, and is moderately resistant to leaf and neck blast. Its average milled rice yield for three years reached 5.34 MT/ha, which is 10% higher than that of ‘Keunnun’. In the brown and germinated brown rice of this cultivar, the total dietary fiber content are 6.45% and 6.71%, and the GABA (γ-amino butyric acid) content are 6.61 and 39.47 mg/100 g, respectively. In addition, the total contents of polyphenol, flavonoid, and tannin were higher in ‘Keunpum’ brown rice than in ‘Keunnun’. The DPPH and ABTS radical scavenging activities were 75.23, 116.08 mg TE/100g, respectively. These functional components and antioxidant activities play an important role in inhibiting diseases and the aging process of the human body. Therefore, it is expected to be used as a basic data in the manufacture of secondary processed products using germinated brown rice (Grant No. 7684).

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휴면기간이 짧고 숙기가 빠른 2기작 감자 신품종 ‘새봉’
‘Saebong’: A Double Cropping Potato Variety with a Short Dormancy Period and Early Maturity
Jang-Gyu Choi, Young-Eun Park, Ju-Sung Im, Young-Gyu Lee, Dong-Chil Chang, Yong-Ik Jin, Jin-Hee Seo, Hyun-Jin Park, Gyu-Bin Lee, Ji-Hong Cho
Korean. J. Breed. Sci. 2021;53(4):526-533.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.526

‘Saebong’ is a double cropping potato variety bred by the Highland Agriculture Research Institute, NICS, RDA, in 2010. Although the major double-cropped potato cultivar of Korea, cv. Dejima, with a dormancy period of 50-60 days after harvest was introduced in 1978, it is susceptible to physiological disorders, such as abnormal tubers and cracking, and common scab. The ‘Chubaek’ variety matures very early and has a dormancy period similar to that of cv. Dejima, yet it is highly susceptible to potato virus Y and has a low dry matter content. ‘Saebong’ was obtained by crossing ‘Daegwan2-8’ with ‘DH9-231’ in 2002. Seedling and line selections were performed from 2003-2004, and yield trials were carried out from 2005-2006 in Gangneung and Pyeongchang. Regional yield trials were conducted in spring and autumn in Namhae, Jeju, and Gangneung from 2007-2008 and in Muan, Jeju, and Gangneung in 2009. This variety produces many white flowers and berries, narrow green leaflets and petioles, and round tubers with beige-colored skin and white-colored flesh. Its dormancy period, when stored at 20℃, was 50-60 days after harvest in spring, similar to cv. Dejima and Chubaek. The average tuber yields in spring and autumn regional trials were 40.4 and 30.5 ton⋅ha-1, respectively. It is susceptible to common scab but highly resistant to potato leafroll virus and potato virus Y. Its dry matter content was 21.56% and 20.60% in spring and autumn, respectively (similar to those of cv. Atlantic and cv. Goun). It produces brighter colored potatoes and can be used to process potato chips and table stock after autumn cultivation in the potato double cropping area of Korea (Registration No. 4873).

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안토시아닌 고함유 자색옥수수 ‘색소1호’의 주요특성
Major Characteristics of Anthocyanin-Rich Purple Corn Hybrid Variety: ‘Saekso 1’
Hee-Yeon Kim, Jong-Yeol Park, Ki-Jin Park, Si-Hwan Ryu, Eun-Ha Chang, Byeong-Dae Goh, Byeong Sung Yoon, Woo Sik Yong, Jae-Keun Choi
Korean. J. Breed. Sci. 2021;53(4):534-538.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.534

A new anthocyanin-rich hybrid variety, ‘Saekso 1’, is developed which is characterized by yellow grains, and purple husks and cobs. This variety was produced by crossing two inbred lines, ‘HA1’ (as the seed parent) and ‘HA2’ (as the pollen parent). It was made in 2008/2009 and evaluated in Hongcheon for two years. After evaluation, the selected variety was named ‘Saekso 1’ and was approved for a variety registration in 2014. The anthocyanin content of ‘Saekso 1’ in husk was 10.39±0.09 g/100 g, which was much higher than that of Kangilok (0 mg/100 g). Since regional tests were conducted only in Gangwon province, this variety is recommended only in that region for commercial cultivation. ‘Saekso 1’ is a health food resource for bioactive materials (Registration No. 4967).

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보리호위축병과 도복에 강한 맥주보리 ‘누리맥’
A Malting Barley (Hordeum Vulgare L.) Variety, ‘Nurimaeg’ with Resistance to Barley Yellow Mosaic Virus and Tolerance to Lodging Stress
Young-Mi Yoon, Yang-Kil Kim, Chon-Sik Kang, Kyong-Ho Kim, Jong-Chul Park, Tae-Il Park, Jin-Cheon Park
Korean. J. Breed. Sci. 2021;53(4):539-547.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.539

A malting barley variety, ‘Nurimaeg’, was developed with resistance to barley yellow mosaic virus (BaYMV), tolerance to lodging stress, and good brewing quality. ‘Nurimaeg’ was developed by crossing Milyang127 and Miharu gold in 2004. Regional yield trials were conducted for ‘Nurimaeg’ as a breeding line ‘Iksan175’ in four different regions from 2014-2016. It has a type I growth habit, and its average heading and maturity dates were April 12 and May 20, respectively-which were 1 day later than the maturity date of ‘Hopum’ variety. As ‘Nurimaeg’ has a shorter culm length (77 cm) than that of ‘Hopum’, it showed tolerance to lodging stress. This variety possesses the rym5 gene and is resistant to BaYMV. It showed a 42.9 g 1000-grain weight and 90% assortment ratio. The yield potential of ‘Nurimaeg’ was 408 kg/10a, which is approximately 18% higher than that of ‘Hopum’. Analysis of grain quality for malting showed that ‘Nurimaeg’ has high quality of low protein and β-glucan content. Malt quality analysis revealed that it has 72.8% extract, 4.2% soluble protein, 38.9% kolbach index, and 243 WK diastatic power, which all were similar to those of ‘Hopum’. ‘Nurimaeg’ would be suitable for cultivation in the areas of Korea that have a daily minimum temperature of -4℃ in the month of January (Registration No. 7960).

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