The purpose of this study was to characterize the T-DNAs introduced into the transgenic OsCK rice, as part of a biosafety evaluation. Choline Kinase (CK) gene is upregulated in the transgenic OsCK rice. We identified the insertion sites, flanking sequences, structures and sequences of the inserted T-DNAs. Based on the adaptor-ligation PCR, we found that the right border of the T-DNA was inserted at position no. 129971, on BAC clone OSJNBa0014J14 of chromosome 10. The flanking sequences of the left border region of the T-DNA (which was later identified as a region harboring a 1-kb long deleted sequence), could not be identified by various PCR-based trials. However, it was finally identified with whole-genome shotgun sequencing, using an Illumina sequencer. The result indicated that one of the T-DNAs was inserted into the CaMV 35S promoter region, whereas the other T-DNA was introduced at position 128947 on OSJNBa0014J14 clone, with an inverse orientation. During the insertion process, a 1024-bp-long chromosome sequences flanked by the right border of the T-DNA region was deleted. A 370-bp long left border region and 199-bp long right border region corresponding to the matrix attachment region (MAR) sequences of the T-DNA were also deleted. Collectively, these results indicate that whole-genome shotgun sequencing is a useful tool to reveal the detailed sequences and structures of the introduced T-DNAs, especially in the case of multiple T-DNA insertions. The expenses incurred on genome sequencing can be easily compensated by minimizing the time and efforts invested in conventional molecular analyses.

This study was conducted to develop breeding lines carrying bacterial blight resistance genes, Xa2, Xa3, xa5, and Xa21 for enhancing the resistance of early maturing glutinous japonica rice. Resistant lines were developed by backcrossing into early maturing glutinous elite cultivar, Sangjuchalbyeo and phenotypic selection for bacterial blight resistance and agronomic traits. Selected lines were confirmed the incorporation of the resistance genes using DNA markers and evaluated the resistance reaction against four Korean and eleven Philippine bacterial bight races. HR24465 line carrying Xa2 was resistant to Korean races K1, K2, and Philippine race 9a. HR24666 line carrying Xa3 was moderately resistant or resistant to races except for Korean race K3a and Philippine race 6. Resistant lines carrying xa5 were resistant to all races except for Philippine race 6. HR24669 line carrying Xa21 was moderately resistant or resistant to races except for Korean race K1 and Philippine race 10. Yield-related traits of breeding lines exhibited similar characteristics to Sangjuchalbyeo. These resistant lines could be useful breeding materials for improving the resistance of early maturing glutinous japonica rice against Xanthomonas oryzae pv. oryzae.

Doubled haploid (DH) system is an effective tool in improving breeding efficiency and has been widely applied in wheat breeding programs. Wheat x maize hybridization is used for the production of wheat DH because of its efficiency and ease of application. We carried out an experiment to investigate genotype effect of wheat (Triticum aestivum) and maize (Zea mays) on efficiency of wheat haploid production. In various wheat x maize crosses, ten wheat and seven maize genotypes were tested. Haploid embryos were rescued and cultured for plant regeneration. Average seed set, embryo formation and haploid regeneration of five wheat varieties in crosses with a pollinator cv. Gangdaok were about 75%, 19% and 8.4%, respectively. Their haploid regeneration ranged from 3.7~9.8% and cv. Jokyoung showed highest regeneration. Average seed set, embryo formation and haploid regeneration of seven maize genotypes in crosses with a parent cv. Jokyoung were about 78%, 18% and 9.6%, respectively. Their haploid regeneration ranged from 7.6~12.9% and cv. Kwangpyeongok showed highest regeneration followed by cvs. Gangdaok and Gangilok. Analysis of variance for seed set and embryo formation showed highly significant effects of wheat parents and maize pollinators, whereas their interaction effect was only significant for seed set. The effect of maize genotypes on these traits was greater than that of wheat genotypes. Consequently, cvs. Kwangpyeongok, Gangdaok and Gangilok were found to be better pollen donors among the genotypes tested in wheat x maize hybridization for wheat hybrid production.

Potato glycoalkaloids (PGAs) are potentially toxic to humans at high levels, and current safety regulations have recommended that PGAs content in tubers of potato cultivars should not exceed 20 mg/100gFW. Accordingly, it is important to determine the PGAs composition and level on potato cultivars for food safety and the breeding for new cultivars with low levels of PGAs. The main aim of this study was to evaluate α-chaconine, α-solanine, and total PGAs content in the peel and cortex portions in four recent cultivars (‘Haryoung’, ‘Goun’, ‘Hongyoung’ and ‘Jayoung’), released by Highland Agriculture Research Center, together with ‘Superior’ and ‘Atlantic’. The total PGAs ranged from 16.5 to 47.7 mg/100gFW. The α-solanine/α -chaconine ratio was 1:3.2 (‘Jayoung’), 1:3.5 (‘Hongyoung’) and 1:2 (‘Superior’), whereas α-solanine was not detected in ‘Goun’, ‘Atlantic’ and ‘Hongyoung’ under the analytical condition of this experiment. 75-94% of total PGAs was existed in the peel part of all cultivars. We selected two cultivars (‘Atlantic’ and ‘Goun’) showing lower PGAs content in the cortex part. During storage at 4°C, total PGAs content fluctuated widely in early stage of storage and stabilized gradually over time in ‘Hongyoung’, ‘Superior’ and ‘Jayoung’. Thus, through the selection of cultivars and storage conditions, these results will provide consumers and breeders with fundamental information about the content of PGAs in Korea major cultivars.

Plant regeneration protocols via adventitious shoot organogenesis from leaf segments of Chrysanthemum morifolium ‘Baekma’ were developed. The effects of plant growth regulators (BA, NAA, IBA, IAA and 2,4-D) and AgNO₃ were tested to figure out the optimal condition for shoot bud induction and shoot formation from leaf explants. On the combination treatment of plant growth regulators and AgNO₃, bud induction was obtained but shoot formation was not. Therefore, two-stage treatment of leaf explants was subsequently experimented for the respective improvement of adventitious bud induction and shoot formation. When leaf explants were cultured on bud induction medium (MS medium supplemented with 0.2 mg/L BA, 1.0 mg/L IAA and 1.0 mg/L 2,4-D) for 4 weeks in darkness and were transferred to shoot formation medium (MS medium supplemented with 1.0 mg/L BA, 1.5 mg/L IBA and 5.0 mg/L AgNO₃) for 4 weeks under the 16/8h photoperiod condition, shoot formation efficiency reached up to 50.0% and 1.6 shoots per explant were acquired. At 12 weeks after culture, the regenerated shoots were elongated and rooted on MS medium. The plantlets were acclimatized successfully and the regenerated plants exhibited normal phenotypes.

This paper compares selection efficiency for high palatability breeding lines using marker-assisted selection and conventional selection methods in rice. A total 4 cross combinations of japonica cultivars were selected using marker-assisted selection with a set of 13 DNA markers associated with grain quality and conventional selection methods in F₃ and F₅ generations assessing palatability using the Toyo taste meter. The multiple regression value with a set of 13 DNA markers was utilized as the marker-assisted selection index. The number of polymorphic markers among 13 DNA markers ranged from 3 to 7 between the parental cultivars. Among these cross combinations, there was no significant difference between marker-assisted selection and conventional selection methods for selection of lines with high palatability. This demonstrates that marker-assisted selection by marker-based regression value might not be a good method for selection to apply the all breeding populations for high palatability line selection. While each method allowed equally effective selection of high palatability lines, the regression analysis using polymorphic markers will need to be re-calculated for each cross combination.

‘Joongmo2003’, a winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross ‘SW86054/Keumkang’ during 1997. ‘Joongmo2003’ was evaluated as ‘Iksan318’ in advance yield trial test in 2006. It was tested in the regional yield trial test between 2007 and 2009. Its heading date was April 24 in upland and April 21 in paddy filed conditions, which was two days earlier than those of the check cultivar ‘Keumkang’, respectively, and maturity date was June 4 in upland and June 2 in paddy field, one or two days earlier than those of ‘Keumkang’, respectively. ‘Joongmo2003’ showed resistance to winter hardiness and pre-harvest sprouting, which lower withering rate on the low ridge (0.3%) and rate of pre-harvest sprouting (18.5%) than ‘Keumkang’ (9.2 and 25.8%, respectively). ‘Joongmo2003’ showed similar protein content (13.2%), lower SDS-sedimentation volume (54.0 ml) and higher gluten content (11.7%) than ‘Keumkang’ (13.1%, 57.8ml and 10.8%, respectively). It showed higher lightness (89.85) of flour and noodle dough sheet than ‘Keumkang’ (89.81 and 80.28, respectively). ‘Joongmo2003’ exhibited similar hardiness (3.84N), springiness and cohesiveness of cooked noodles (0.92 and 0.63) compared to ‘Keumkang’ (4.54N, 0.93, and 0.63, respectively). The average grain yield in the regional yield trial was 555 kg/10a in upland and 485 kg/10a in paddy field, which were 4% and 7% lower than those of the check cultivar, ‘Keumkang’, respectively.

‘Joongmo2004’, a winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross ‘Bacanora88/Keumkang//Keumkang’ during 1996. ‘Joongmo2004’ was evaluated as ‘Iksan320’ in advance yield trial test in 2006. It was tested in the regional yield trial test between 2007 and 2009. Its heading date was April 26 in upland and April 23 in paddy filed conditions, and Maturity date was June 5 in upland and June 4 in paddy field, which were similar to Keumkang, respectively. It showed 830 in number of spikes per m², 35 of grain number per spike, 44.9g of 1,000 grain weight, and 806g of test weight. ‘Joongmo2004’ showed moderate to fusarium graminearum (Scab) in test of specific character although ‘Keumkang’ is susceptible to scab. ‘Joongmo2004’ had higher flour yield (75.5%) than ‘Keumkang’(72.5%). ‘Joongmo2004’ showed similar ash (0.42%), lower protein content (12.3%), SDS-sedimentation volume (43.0 ml) and gluten content (10.3%) than ‘Keumkang’ (0.42%, 13.1%, 57.8ml and 10.8%, respectively). It showed higher lightness (89.97), redness (-1.38) and yellowness (10.76) in flour color than ‘Keumkang’ (89.61, -1.15 and 9.46, respectively). ‘Joongmo2004’ exhibited lower hardness (3.50N), similar springiness and higher cohesiveness of cooked noodles (0.92 and 0.65) compared to ‘Keumkang’ (4.54N, 0.93, and 0.63, respectively). The average grain yield in the regional yield trial was 517 kg/10a in upland and 534 kg/10a in paddy field, which were 11% lower and 3% higher than that of the check cultivar, ‘Keumkang’, respectively.

Cymbidium ‘Sky Pink’ (Lucky Rainbow ‘Randezvous’ x Lucky Rainbow ‘Lapin Hot’) was developed from a cross between hybrids at the National Institute of Horticultural & Herbal Science (NIHHS), Rural Development Administration (RDA) in 2008. A cross was made between pink colored flower C. Lucky Rainbow ‘Randezvous’ as maternal line and bright pink colored flower, C. Lucky Rainbow ‘Lapin Hot’ as paternal line in 1995. The seed germination, cultivation, selection, and characteristic trials were conducted from 1996 to 2002. The line was named as Wongyo ‘F1-23’ and phenotype was characterized in 2007 as a new cultivar. The ‘Sky Pink’ has bright pink basal color (RHS, RP63C) on both of sepal and petal with red lip (RHS, RP59A). This variety has about 11.4 flowers per flower stalk and flower size of 6.3cm. General appearance of petals and sepals is slightly incurved shape. The plant size is intermediate having erect peduncle. Blooming is started from the middle of January (mid-winter) under optimal culture condition. Leaf attitude and twisting is erect and weak respectively. This hybrid has attractive floral arrangement, long flower stalk (53.0cm) and vigorous growth. We expect that ‘Sky Pink’ has a great potential for exporting to Chinese market. Blooming life of a 'Sky Pink' variety as pot flower was about 60 days and that was 18 days as cut flower under room temperature (18-23°C). This new variety was suitable for using as a cut and pot flower.

A soybean cultivar for sprout, ‘Joyang 1’ was developed at the National Institute of Crop Science (NICS) in 2010. ‘Joyang 1’ (Iksan67) was released by pedigree selection from the cross between high yielding ‘Pungsannamulkong’ and ‘Suwon187’ tolerant to lodging and disease. It has purple flower, lanceolate leaf, grey pubescence and small yellow seed. Weight of hundred seed is 11.6gram. Maturing date of ‘Joyang 1’ is ten days earlier than check cultivar, ‘Pungsannamulkong’. ‘Joyang 1’ is resistant to bacterial pustule and shattering. It have similar tolerance to root rot and seed spot to check cultivar. It shows high germination ratio, less abnormal germination and hard seed. Sprout yield also 11 percent higher than check cultivar. The average seed yield of ‘Joyang 1’ was 3.03 ton per hectare in the regional yield trials (RYT) carried out for three years from 2008 to 2010 which was 3 percent higher than the check cultivar. The number of breeder’s right is ‘4900’.

‘Jungmo 7001’ is a intermediate parent of rapeseed (Brassica napus L.) with wide adaptable, early flowering and large flower for the scenery and production of edible oil. ‘Jungmo 7001’ was developed from the cross between ‘[(Mangun × Bronowski) × Erra] × Tower’ as female parent and ‘RS10’ as male parent in 1984. ‘Jungmo 7001’ has green and middle parted leaf, yellowish flower and black seed coat. The flowering and ripening period of ‘Jungmo 7001’ were 3 days earlier than check variety 'Hanlayuchae'. The average flower size of ‘Jungmo 7001’ was significantly greater than flowers of 'Hanlayuchae'. The average seed yield of ‘Jungmo 7001’ was higher compared with 'Hanlayuchae' in local adaptability test. Total oil content of ‘Jungmo 7001’ was 44.4%. In fatty acid composition, ‘Jungmo 7001’ has no erucic acid. However, oleic acid content is 69.1%, which is 1.7% higher than 'Hanlayuchae'. ‘Jungmo 7001’ will be adaptable to southwestern area including Jeonllanam-do, Jeollabuk-do and Gyeongsangnam-do and Jeju island of Korea.

A new peanut variety ‘Jaseon’ (Arachis hypogaea ssp. fastigiata.) was developed by the Department of Functional Crop, NICS, in Milyang in 2011. This was developed from the cross between the large grain cultivar ‘YG60’ and the short stem cultivar ‘YG55’. ‘Jaseon’, which is a Shinpung plant type, has 11 branch number and 43 cm in main stem length. Each pod has two grains with brown testa and long-ellipse shaped large kernel and its 100 seed weight was 98 g in the regional yield trials (RYT). ‘Jaseon’ showed 43.2% of crude oil and 43.0% of oleic acid composition in dry peanut, and had 25.0 mg/g of Sucrose and 4.4 mg/g of tannin content in fresh peanut. In the regional yield trials, ‘Jaseon’ was outyielded by 15% with 10.21 MT/ha for fresh pod and by 11% with 4.37 MT/ha for dry grain, compared those of check variety.

‘Hwaweon3’ was developed from an interspecific cross between Hwayeongbyeo and a wild species, Oryza rufipogon L. (W1944) based on marker-aided selection and backcrossing. The recurrent parent ‘Hwayeongbyeo’ is a high grain quality cultivar with medium-maturity. ‘Hwaweon3’ is nearly isogenic to Hwayeongbyeo except a small O. rufipogon introgression on chromosomes 1, 8 and 12 harboring the QTL gene for cold tolerance. The preliminary and replicated yield trial was conducted at Chungnam National University in 2006 and 2007. The local adaptability test was carried out by the National Seed Management Office (NSMO) in 2008 and 2009. This cultivar was registered to NSMO with a cultivar designated as ‘Hwaweon3’. This cultivar averaged 78 cm in culm length and has a medium growth duration. This variety is resistant to stripe virus as the recurrent parent, and moderately resistant to leaf blast disease with durable resistance. Milled rice of ‘Hwaweon3’ is translucent and the grain quality traits are comparable to those of the recurrent parent. it has low protein content. The yield potential of ‘Hwaweon3’ in grain is about 7.23 MT/ha at the ordinary fertilizer level comparable to that of Hwayeongbyeo. ‘Hwaweon3’ performed better than Hwayeongbyeo in cold tolerance-related trait in the cold water-irrigated plot in the Chuncheon sub-station. This is mainly due to the introgressed segment from O. rufipogon on chromosome 1 harboring pe1.2 for panicle exsertion. ‘Hwaweon3’ could be used as a bridging line to enhance cold tolerance of japonica breeding lines.

‘Cheongpoongbora’, a winter hairy vetch variety (Vicia villosa Roth) for green manure use, was developed from the National Institute of Crop Science (NICS) in 2009. Among the genetic resourced collected in Daegu, early flowering, strongly resistant to cold, and high yield in green manure, was selected by pedigree selection method. Over the two years from 2008 to 2009, regional yield trial test was conducted with the line in four regions: Suwon, Iksan, Milyang, and Yeongdeok. As a result, in December 2009, it was named ‘Cheongpoongbora’ as a new variety. ‘Cheongpoongbora’ features medium green color leaves, medium density hair, and black seed coat. Flowering season for the variety was on April 29 on average across the nation, 11 days earlier than the check variety ‘Welta’. Also, it was more resistant to cold than ‘Welta’. The dry matter yield was average 5.20 tons per ha, 5.9% higher than ‘Welta’ (4.92 tons), the nitrogen yield was average 166 kg per ha, 6.4% higher than ‘Welta’ (156 kg). ‘Cheongpoongbora’ can be cultivated in any national regions which have the daily minimum mean temperature of over -15°C in January.

A new naked oat (Avena sativa L.) cultivar ‘Suyang’ was developed by National Institute of Crop Science, RDA in 2010. It was derived from the cross between ‘Sikyonggwiri’ and ‘Gwiri23’. Subsequent generations were handled in a bulk method and pedigree selection program, and ‘SO96025-B-303-44-2-4’ was selected for agronomic appearance in 2002. The line showed both high yield and good husking rate of seed in the Yield Trial tested at Suwon in 2003 to 2004, being designated as ‘Gwiri56’. The ‘Gwiri56’ was subsequently evaluated for winter hardiness, earliness, and yield in four locations, Gimje, Iksan, Jeongeup and Jinju from 2005 to 2010 and was released as the name of ‘Suyang’. ‘Suyang’ had 11 days earlier heading date and 6 days earlier maturing time than the check cultivar ‘Seonyang’ in field condition. The ‘Suyang’ had 23.7 cm of spike length, 568 spikes per m², 73 grains per spike, 29.2 g of 1,000-grain weight, and 646 g of test weight. ‘Suyang’ showed better winter hardiness than that of the check cultivar ‘Seonyang’, and similar seed quality to the check cultivar in respect to crude protein (11.3%) and β-glucan content (4.7%). However, it showed higher husking rate than that of check cultivar. The grain yield of ‘Suyang’ in the regional yield trial for 4 years was averaged 4.35 MT ha^-1, which was 29% higher than that of the check cultivar ‘Seonyang’. ‘Suyang’ is recommended for fall sowing cropping only in the south area where daily minimum mean temperatures are averaged higher than -4°C in January.

Soybean is the main source of protein and oil for human and animal nutrition. However, antinutritional factors in the raw mature soybean are exist. Lipoxygenase protein is the main antinutritional factor in mature soybean seed. A new soybean cultivar, ‘Jinyang’ with yellow seed coat and free of lipoxygenase 1,2,3 protein was developed. It was selected from the population derived from the cross between ‘Jinpumkong2ho’ and GS301. ‘Jinyang’ has purple flowers, tawny pubescence, a determinate growth habit, oval leaflet shape, and light brown pods at maturity. The seed has yellow hilum and yellow seed coat color. Seed protein and oil content on a dry weight basis were 33.5% and 16.6%, respectively. It has shown a resistant reaction to soybean necrosis, soybean mosaic virus, cercospora leaf spot and blight, black root rot, pod and stem blight, and soybean pod borer. ‘Jinyang’ matured in 1 October with plant height of 65cm and a 100-seed weight of 21 g. Average yield of ‘Jinyang’ was 300 - 330 kg/10a in 2010 - 2012. ‘Jinyang’ has been registered as a new soybean cultivar (registration number: 4279, registration date: Dec. 28, 2012) by Korea Seed & Variety Service, Republic of Korea.