Arabinoxylans (AX) are hydrophilic nonstarch polysaccharides found in wheat grain that affects end-use qualities,including bread-making quality. The objectives of this study were to (1) investigate the effects of wheat cultivar, growingconditions and locations on AX content; (2) evaluate AX content on the comparison of the ten Korean and CIMMYT wheatcultivars; and (3) determine the correlations between AX content and bread-baking quality to improve nutrition of baking quality.Water-extractable arabinoxylan (WE-AX) contents, total arabinoxylan (TO-AX) contents, and the percentage of WE-AX contentsover TO-AX contents (WE-AX/TO-AX) were analyzed. Twenty-six Korean winter wheat cultivars were cultivated in twolocations for three consecutive years. The results indicated that WE-AX, TO-AX, and WE-AX/TO-AX were significantlyinfluenced by genotypes, year and the interactions between year and genotype. Keumkang showed lower WE-AX content (3.25mg/g) than other Korean wheat cultivars. Eunpa, Jinpoom, Seodun, and Shinmichal 1 exhibited the higher TO-AX content(>18.65 mg/g) than other cultivars and Jokyoung and Keumkang (

The objective of this study was to evaluate the suitability of simple sequence repeat markers for variety identificationin 69 rose (Rosa x hybrida) varieties. A set of 112 SSR primer pairs was evaluated and 43 primer pairs showed polymorphismin 12 varieties. Twenty-two primer pairs out of 43 primer pairs showed high levels of polymorphism and reproducibility. Thegenetic relationship of 69 varieties was analyzed based on the marker genotypes of 22 SSRs. A total of 114 polymorphicamplified fragments were obtained by using 22 SSR markers. Two to ten SSR alleles were detected for each locus with anaverage of 5.18 alleles per locus. Average polymorphism information content (PIC) was 0.621, ranging from 0.211 to 0.813. Atotal of 114 marker loci were used to calculate Jaccard’s distance coefficients for cluster analysis using unweighted pair-groupmethod with arithmetical average (UPGMA). Cluster analysis of genetic diversity revealed that these SSR marker sets identifiedeach genotypes of 69 rose varieties. These SSR markers may be used for wide range of practical application in varietyidentification of rose.

Tomato shelf-life is important for its fresh market usage especially for exporting and transporting tomato in tropicalclimate. To increase the shelf-life, delaying maturity is one of the powerful methods. A ripening gene related to the fruit maturitydelaying was previously known through tomato mutation. RIPENING-INHIBITOR (Rin) from the recessive mutation effectivelyblocks the ripening process, and when the hybrid (Rin/rin) was formed it shows slow-ripening and long shelf-life. Since breedersneed the long shelf-life character in the tomato breeding program, we had set out a series of experiments to develop a Rin relatedDNA marker. We searched for the proper primer sequences in the deletion region between Rin and rin mRNA sequences, andalso in the intron region of rin. Primers that simultaneously amplified two bands with a co-dominant pattern were selected andconverted to the Sequence Characterized Amplified Region (SCAR) marker. As a preliminary data, the SCAR marker clearlydistinguished genotypes between heterozygote (Rin/rin) and homozygote (Rin/Rin, rin/rin), and this would help select the tomatolines that have a characteristic of long shelf-life.

The purpose of this research is a molecular characterization of introduced T-DNA in transgenic rice T4~T6 generationlines (#8) harboring a pepper MsrB2 gene under the control of stress inducible Rab21 promoter, as a part of biosafety evaluationfor drought-tolerant transgenic rice (CaMsrB2-8). We identified the structure and sequence of transformation vector of T-DNAand analyzed insertion sites, flanking sequences, and generational stability of inserted T-DNA in transgenic rice. The transformationvector was consisted of right border, a drought-tolerant CaMsrB2-8 gene unit (Rab21 promoter::CaMsrB2::PinII terminator), aselectable marker herbicide resistance unit (CaMV 35S promoter::bar::Nos terminator), and left border in sequential order. Weconfirmed that T-DNA was introduced at the position of 41,737,284 bp of chromosome No. 1. based on the adaptor-ligation PCRand whole genome sequence database. T-DNAs were stably inherited through the T4 to T6 generations, and also stable expressionof bar gene from T-DNA was confirmed. It was also confirmed that the backbone DNA of transformation vector containingantibacterial gene (aadA) was not present in CaMsrB2-8 rice genome. These results will be filed to biosafety assessment documentof CaMsrB2-8 rice.

This study was conducted to investigate the resistance reaction of single resistance genes and their pyramiding effectsin indica and japonica rice against bacterial blight disease in Korea. Resistance reaction of indica and japonica near-isogeniclines and pyramided lines carrying resistance genes to four predominant and other sixteen bacterial blight isolates was investigated.Among singe resistance genes, Xa1, Xa3, and Xa21 in indica and japonica near-isogenic lines exhibited same or similar resistancereaction. On the other hand, Xa4 and xa5 showed higher resistant reaction in indica background than in japonica background.Combined resistance genes provided the pyramiding effects such as increasing the resistance relative to single gene or two genesand the complementation of susceptibility in both indica and japonica rice. Newly identified combinations in japonica rice,Xa3+Xa21, xa5+Xa21, and Xa3+xa5+Xa21, conferred resistance to all isolates and showed higher resistant reaction than Xa3+xa5widely used in Korea. These could be the efficient and promising combinations for enhancing the resistance of japonica riceand delaying the breakdown of resistance against Xanthomonas oryzae pv. oryzae in Korea.

A new poinsettia (Euphorbia pulcherrima Willd. Ex Klotzch) cv. ‘Grace’ was bred by the National Institute ofHorticultural and Herbal Science (NIHHS) in 2009. A cross was made between ‘PC-025’ and ‘Ichiban’ in 2006. ‘PC-025’ is aline with curled peach color bracts, and ‘Ichiban’ is a cultivar with early coloring bright red bracts. ‘Grace’ was finally selectedin 2009 after the investigation of the growth and flowering characteristics from 2007 to 2009. ‘Grace’ has peach colored ellipticbracts and weak intensity of rugosity between bract veins. The leaf blade of ‘Grace’ has ovate shape and dark green color. Thepetiole length of ‘Grace’ is medium, and stem color is greenish. ‘Grace’ has strong free-branching ability and short plant height.Bracts and transitional leaves of ‘Grace’ are fully colored 7 weeks after short-day commencement.

A new rose cultivar, ‘Purple Dew’ was selected from the progenies of a cross between ‘Boy Friend’ and ‘GSR10315’by rose breeding team of the Gyeonggi-Do Agricultural Research and Extension Services in 2009. ‘Purple Dew’ was crossedin 2006 and seedlings were produced. After the evaluation of specific characters from 2007 to 2009, it was finally selected andnamed. ‘Purple Dew’ has standard flower type with purple color (RHS Color Chart Red Purple Group N66C). The leaf glossinessis strong and it is resistant to powdery mildew. ‘Purple Dew’ grows vigorously, it takes 48 days to flowering and cut flowerproductivity is 164.1 stems/m2 in a year. The length of cut flower is about 70 cm and the fresh weight of cut flower is 39.3g. It has 10.5 cm in flower diameter, 52 in petal numbers per flowers and 12 days in vase life.

A new sesame variety ‘Milsung’ was developed from Yeongnam Agricultural Research Institute, National Institute ofCrop Science, Rural Development Administration in 2007. Cross was made by ‘Iri 4’ and ‘Suwon 131’ with high quality andyields capacity and disease resistance. And ‘Milsung’ was followed by breeding methods of pedigree selection, yield performancetrials and regional yield trials by the sesame breeding team at the National Institute of Crop Science. ‘Milsung’ has a few branchwith tri-capsuled, bi-loculi and quadri-carpellates. And the color of flower is light pink. Flowering date of ‘Milsung’ was Jul1st which was two days earlier than that of the check variety ‘Yangback’. Maturing date of ‘Milsung’ was Aug 20th which wassame to that of the check variety ‘Yangback’. Plant height of ‘Milsung’ was 129 cm which was similar to that of the check.But ‘Milsung’ had 27 capsuled nodes which was more than those of the check. So ‘Milsung’ showed very dense capsuled shape.Disease and disaster resistance including phytophthora blight and lodging were similar to the check. The yield of ‘Milsung’ was98 kg/10a which increased by 5% compared to that of the check. Oil content of ‘Milsung’ was lower than that of the checkbut higher in protein. Oleic acid composition of fatty acid was 45.8 percent. The contents of sesamin is 2.8 mg/g and those ofsesamolin is 3.0 mg/g.

‘Hwaweon 5’ was developed from a cross between the African upland cultivar, ‘Moroberekan’ and ‘Ilpumbyeo’ basedon marker-aided backcross selection. The recurrent parent ‘Ilpumbyeo’ is a high grain quality cultivar with medium to late maturity.‘Hwaweon 5’ is nearly isogenic to ‘Ilpumbyeo’ except for ‘Moroberekan’ introgressed segments on chromosomes 1, 4 and 6.The Moroberekan segment on Chromosome 4 has a resistance gene for blast. The preliminary and replicated yield trial wasconducted at Chungnam National University in 2006 and 2007. The local adaptability test was carried out by the National SeedManagement Office (NSMO) from 2008 to 2009. This cultivar was registered to NSMO designated as ‘Hwaweon 5’. This cultivaraveraged 78 cm in culm length and has medium growth duration. Milled rice of ‘Hwaweon 5’ is translucent and the grain qualitytraits are comparable to those of the recurrent parent. It has low protein content. The yield potential of ‘Hwaweon 5’ in grainwas about 6.53 MT/ha at the ordinary fertilizer level for two years. This variety showed highly resistance reaction at the blastnursery test at four locations and also at the sequential planting method. This resistance is due to the gene designated as Pi45(t)on chromosome 4 from Moroberekan. The Pi45(t) gene will be useful in enhancing resistance to blast in rice breeding program.

‘TY Altorang’ was developed from a cross between ‘MS-CDE’ and ‘VFR’ in 2009. The cultivar has multi-diseaseresistances against Tobacco mosaic virus(TMV), Tomato yellow leaf curl virus(TYLCV), Fusarium crown and root rot and Leafmold disease. Additionally, this cultivar produces larger fruit set resulting in higher fruit harvest. Fruit shape is fair and uniformedovate type with an average fruit weight of 225 g as a beef-steak type tomato, and the fruit presents longer shelf-life due to higherfruit firmness. The early and medium-maturing variety can be transplanted in February to March, or July to September.Medium-strong plant-vigor and low occurrence of creased stem allow, relativey easy cultivation.

　‘Titi Chal’ with elite characteristics and high fruit yield was developed from a cross between ‘MS-BGT’ and ‘NHMF’in 2009. The cultivar has multiple disease resistances against Tobacco mosaic virus, Tomato yellow leaf curl virus, Tomatospotted wilt virus and Leaf mold disease. The early and medium-maturing variety can be transplanted on July to September. Thecherry tomato presents longer shelf-life due to higher fruit firmness and also has high eating quality with 9~10 brix of sugarcontent. The fruit shape is red plum type with an average fruit weight of 23~25 g. The ‘Titi Chal’ is more reliable to be cultivatedbecause of medium-strong plant-vigor and low occurrence of creased stem.

A new soybean cultivar ‘Hoban’ was released by ARES, Gangwon Province in 2007. The Goals of breeding werefor large seed size, high yield, lodging tolerance, and resistance to diseases such as soybean mosaic virus (SMV) and bacterialpustule. ‘Hoban’ was derived from the cross of GWS 91, which has yellow seed coat, large seed size and late maturity, and‘Jinpum’, which lacked two of three lipoxygenase isoenzymes (L-2 and L-3). The preliminary, advanced and regional yield trialsfor evaluation and selection were carried out from 2001 to 2006. ‘Hoban’ was characterized as lipoxygenase 2 free, large seed.It has a determinate growth habit with purple flower, yellow seed coat, yellow hilum and large seed weight(29.0g per 100 seeds).The maturing date of ‘Hoban’ was Oct. 5. The content of total isoflavone was 1,995㎍/g which was 25% higher than checkvariety (‘Jinpumkong 2’). The average yield of ‘Hoban’ was 2.69 MT/ha in the regional yield trials (RYT) carried out in twolocations of Korea from 2004 to 2006 which was 3 percent higher than the check variety ‘Jinpumkong 2’. SDS-PAGE on grainstorage protein shows that ‘Hoban’ was diffcient in lipoxygenase 2.

This study was conducted to develop an inbred parental line with high sugar content and yield in octoploid strawberry.‘Wongyo 3114’ was selected to have predominant horticultural traits including plant vigor, sugar content and yield amongprogenies of ‘Benihoppe’. ‘Wongyo 3114’ shows erect plant type, vigorous growth habit, early flower bud differentiation with16 flowers per cluster. Fruits of ‘Wongyo 3114’ are conical type having a bright red skin color, and 17 g in an average weight.Sugar and acidity content are similar to check cultivar, ‘Akihime’, but the firmness is lower than check cultivar. Yield isrelatively higher than check cultivar, however, its marketable yield is lower than that of check cultivar because of poorfruit-setting under low temperature condition. Disease and pest resistance of ‘Wongyo 3114’ are more sensitive and similar topowdery mildew, gray mold and two-spotted spider mites than check cultivar. Therefore, ‘Wongyo 3114’ is suitable for anintermediate parent line rather than a cultivation cultivar.

A new Saxifraga fortunei cultivar ‘Foxstar’ was bred by crossing between ‘Hongsaja’ with red purple (RPN66A)colored petals and ‘Yoangwoo’ with light pink (RPN57C) colored petals. The selection and flower characterization wereconducted from 2004 to 2008 in greenhouse of Highland Agriculture Research Center, NICS, RDA. The flowering of ‘Foxstar’occurred from October 5 for 28 days, The number of flowers per plant was 160.0 with many flowering habit. The flower showsdark pink (RPN66B) color at the opening, The number of petals were 6.1, with a width of 1.9cm and length of 2.1 cm. Theplant height was 12.6 cm, and number of flower stem was 10.2, and number of leaves per plant was 84.0. ‘Foxstar’ cultivarcan be used for pot flower. Fifty percent shading is a good cultural practice against direct sunlight and soft rot disease wouldoccurs usually by over-irrigation.

A new peanut variety ‘Ilpyeong’ (Arachis hypogaea ssp. hypogaea L.) was developed at the Department of FunctionalCrop, NICS, in Milyang in 2009. This cultivar was developed from a cross between the high-yielding cultivar ‘Palkwang’, andthe high quality cultivar ‘Yudelaka’. ‘Ilpyeong’ which is Virginia plant type has 20 branch numbers per plant with longellipse-shaped large kernel. Each pod has two grains with brown testa and 100 seed weight was 85 g in the regional yield trials(RYT). Seed quality showed 49.5% of crude oil and 27.6% of protein content. This variety also showed more resistance to lateleaf spot and web blotch compared with check one. Especially it has resistance to lodging until harvesting owing to short stemand erect plant type. In the regional yield trials ‘Ilpyeong’ was outyielded than the check variety by 8% with 4.47 MT/ha for grain.

‘Jopyeong’ is a new japonica rice variety developed from a cross between cold tolerant ‘HR16683-46-3-1’ and ricestripe virus and bacterial blight resistant ‘HR18129-B-16-1-4’ by the rice breeding team of Department of Rice and Winter CerealCrop, NICS, RDA in 2010. This cultivar has about 114 days of growth duration from transplanting to harvesting in northernplain, northern and southern mid-mountainous area of Korea. This cultivar is about 67 cm in culm length and tolerant to lodging.‘Jopyeong’ was multiple resistance to rice stripe virus, bacterial blight and blast. The milled rice of ‘Jopyeong’ exhibits translucent,relatively clear non-glutinous endosperm and medium short grain. Compared to ‘Odaebyeo’, it has similar amylose content(19.9%), palatability of cooked rice and high protein content (6.5%). The milled rice yield performance of this cultivar was about5.67 MT/ha in local adaptability test. ‘Jopyeong’ would be adaptable for ordinary transplanting in northern plain, mid-mountainousand southern mountainous area of Korea.

Cymbidium ‘Green Honey’ (Cymbidium ‘A-one’x Cymbidium ‘Oji’) was developed from a cross between hybrids atthe National Institute of Horticultural & Herbal Science (NIHHS), Rural Development Administration (RDA) in 2008. A crosswas made between green colored flower with red spotted lip of medium and small plant type, Cymbidium ‘A-one’ as a maternalline and pure yellow colored flower with yellow lip of small type, Cymbidium ‘Oji’ as a paternal line in 1995. The seedgermination, cultivation, selection, and characteristic trials were conducted from 1996 to 2007. The line was named as Wongyo‘F1-22’ and phenotype was evaluated 2007 as a new cultivar. The ‘Green Honey’ has light green basal color (RHS, G142A)on both of sepal and petal with red spotted lip (RHS, R53A). ‘Green Honey’ has about 11.2 flowers per flower stalk and flowersize of 6.8 cm. General appearance of petals and sepals is slightly incurved shape. The plant size is medium and half-erectpeduncle. Blooming was started from the middle of November (early winter) under optimal culture condition. Leaf attitude andtwisting is erect and weak respectively. We expect that ‘Green Honey’ has a great potential for domestic market due to thishybrid has attractive flower color, short flower stalk length (51.5 cm) and vigorous growth.